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77.7K Views
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10:00 min
March 24th, 2014
DOI :
10.3791/51100-v
Chapters
0:05
Title
1:25
Activation of NK Cell on Antibody-coated Coverslips
3:19
Fixing, Permeabilizing, and Staining NK Cells on Coverslips
4:52
Mounting the Coverslips
5:35
STED Imaging
8:21
Optimizing STED Imaging: F-actin in NK Cells
9:30
Conclusion
Summary
在这里,我们示出了协议,用于成像由两色STED纳米显微NK细胞扼要重述在玻璃上的细胞毒免疫突触。使用这种方法,我们获得的突触蛋白和细胞骨架的亚100纳米分辨率。
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