Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
77.7K Views
•
10:00 min
March 24th, 2014
DOI :
10.3791/51100-v
Chapters
0:05
Title
1:25
Activation of NK Cell on Antibody-coated Coverslips
3:19
Fixing, Permeabilizing, and Staining NK Cells on Coverslips
4:52
Mounting the Coverslips
5:35
STED Imaging
8:21
Optimizing STED Imaging: F-actin in NK Cells
9:30
Conclusion
Summary
ここでは、2色STEDナノスによってガラス上で要約NK細胞の細胞傷害性免疫シナプスを撮像するためのプロトコルを示す。この方法を用いて、我々は、サブ100nmシナプスタンパク質の分解能と細胞骨格を得た。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved