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DOI :
10.3791/51114-v
January 17th, 2014
Chapters
0:05
Title
1:44
Preparation of the Bacterial Suspension
3:07
High-throughput Genome-wide siRNA Screening
6:16
High-throughput Compound Screening
8:27
Image Acquisition and Analysis
11:10
Results: Mtb Colonization in Host Cells is Inhibited by Silencing Dectin-1 and by Isonazid or Rifampicin Treatment
14:37
Conclusion
在这里,我们描述了一种表型检测,适用于小干扰合成RNA(siRNA)、化合物和 结核 分枝杆菌突变库的高通量/高含量屏幕。该方法依靠使用自动共聚焦显微镜检测荧光标记的荧光结 核分枝杆菌 。
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