Name: a fully automated rodent conditioning protocol for sensorimotor integration and cognitive control experiments
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所有涉及动物的程序批准了美国密歇根州立大学实验动物管理和使用委员会（IACUC）。 1，实验装置<ol><li>使用操作条件反射箱，由一个五孔nosepoke墙一侧和另一侧一个送外卖的低谷。 <ol><li>该中心nosepoke孔被认为是一个“固定”孔和其他四个孔（两个固定孔的每一侧）被认为是电动机目标孔。每个孔都配有一个三色LED和红外光束发射器 - 检测器系统，当动物进入和从固定孔?...

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大鼠已广泛应用于神经科学的研究了一个多世纪。由于桑代克的引进效果猫34法的概念，操作条件一直是标准的方法来测试动物的行为的不同方面。涉及决策和运动准备许多神经科学实验，包括指令线索和动作间隔之间的延迟时间。这是可取的，在这延迟期间尽量减少运动，以减少任何的困惑，以被收购神经的数据。而传统的迷宫导航实验啮齿动物的利用啮齿类动物的巨大能力，觅食，它...

<html>研究神经生理学和行为之间的关系是系统神经科学的终极目标。在历史上，出现了动物模型的选择和行为剧目1-5之间的权衡。而简单的生物如海蛞蝓6或鱿鱼7已被广泛用于研究单个离子通道，神经元和简单的神经回路的性能，需要更高阶的物种，研究更复杂的功能，诸如空间导航，决策8-11和认知控制12-14。尽管是一个标准的动物模型，为人类行为一样，使用非人类灵长类动物的提示，排除其使用在广泛的实验，化验单设置15-18成本和伦理方面的考虑。简单的动物模型，如啮齿类动物，一般首选19，只要有利益的行为基本相似的神经基础。 “&gt;有充足的证据表明，啮齿动物有着相似的皮层和皮层下结构与在灵长类20-22找到。鼠害也被称为跨多个感官的方式整合信息来指导自己的行动23-25，例如，通过协调搅打和嗅探在探索行为26或通过整合听觉和视觉/嗅觉事件25,27。 在这里，我们描述了用于测试的认知任务28-32啮齿动物的操作性条件反射的框架。在这个框架中，受试者被要求注视一个nosepoke孔内并维持其吻部的孔内，直到一展身手提示的呈现。的行为的任务是常规用于5 - 选择序列反应时任务的研究五孔nosepoke设计。在延迟期间，一系列指令线索呈现给引导的主语要执行的操作。这个框架可以很容易地修改，以适应需要广泛的实验，其中训练受到了一个短暂的间隔尽量减少其明显的运动。这允许研究在何种程度上在此间隔内的单个神经元的尖峰活动是受特定的线索。该协议可以减少培训时间，可以减少跨学科学习的变异。该任务的流程示意图示于图1。

<table border="0" cellpadding="0" cellspacing="0" width="757">
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			<td height="42" style="height:42px;width:359px;"> 5-HOLED NOSE POKE WITH 3STIM CUE LIGHT - RAT CAGE</td>
			<td style="width:112px;">Coulbourn</td>
			<td style="width:119px;"> H21-06M/R</td>
			<td style="width:167px;"> </td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:359px;">TEST CAGE</td>
			<td style="width:112px;">Coulbourn</td>
			<td style="width:119px;">H10-11R-TC</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:359px;">Graphic State Software</td>
			<td style="width:112px;">Coulbourn</td>
			<td style="width:119px;"> </td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:359px;">PROGRAMMABLE TONE/NOISE GENERATOR</td>
			<td style="width:112px;">Coulbourn</td>
			<td style="width:119px;">A12-33</td>
			<td></td>
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			<td height="21" style="height:21px;width:359px;">Dustless Precision Pellets</td>
			<td style="width:112px;">Bio-Serv</td>
			<td style="width:119px;">F0165</td>
			<td></td>
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			<td height="21" style="height:21px;width:359px;"> SPEAKER MODULE</td>
			<td style="width:112px;">Coulbourn</td>
			<td style="width:119px;">H12-01R</td>
			<td></td>
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a fully automated rodent conditioning protocol for sensorimotor integration and cognitive control experiments

啮齿动物已被传统上用作涉及的感官，认知和运动任务无数实验室实验的标准动物模型。需要精确控制感觉反应，如决策和注意调控较高的认知功能，但是，通常在评估非人类灵长类动物。尽管灵长类动物的行为，使这些功能加以研究多个变种的丰富性，啮齿动物模型仍然是一个有吸引力的，具有成本效益的替代灵长类动物模型。此外，完全自动化操作条件在啮齿类动物的能力，在非人灵长类动物的劳动密集的训练增加了独特的优势，在研究范围广泛的这些复杂的功能。 在这里，我们介绍一个协议operantly调节大鼠上进行工作记忆任务。在任务的关键时期，该协议确保了动物的显性运动被最小化，热曲爱菱动物为&#39;凝视&#39;，直到转到线索传递，类似于非人灵长类实验设计。一个简单的两种可供选择的被迫选择任务的实施是为展示了其性能。我们讨论这个范例的应用到其他任务。

建议的框架使培训对象在一系列的认知任务。在这里，我们实现了一个指令延迟任务旨在调查在啮齿动物前额叶皮层目标导向动作的机制。 图1显示了实验设计的流程图。 为了确保受试者理解任务要求，步步为营，绩效指标应持续评估。 图2显示了在多个会话一个课题的例子表现。一旦受试者获取的任务，它被植入的前度区域的32通道微电极阵列（对?...

Watch this Scientific Journal Video about A Fully Automated Rodent Conditioning Protocol for Sensorimotor Integration and Cognitive Control Experiments at JoVE.com

A Fully Automated Rodent Conditioning Protocol for Sensorimotor Integration and Cognitive Control Experiments

一种啮齿动物操作性条件反射完全自动化的协议被提出。该协议依赖于行为事件的精确时间控制，调查的程度，这种控制影响神经活动基本感觉整合和认知控制实验。

为感觉运动整合与认知控制实验完全自动化的啮齿动物调理协议

Rodents have been traditionally used as a standard animal model in laboratory experiments involving a myriad of sensory, cognitive, and motor tasks. ...

Research

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Behavior

A Method for Investigating Age-related Differences in the Functional Connectivity of Cognitive Control Networks Associated with Dimensional Change Card Sort Performance

针对调查中的认知控制网络与关联维度变化卡片分类性能的功能连通性年龄相关性差异的方法

The ability to adjust behavior to sudden changes in the environment develops gradually in childhood and adolescence. For example, in the Dimensional ...

科研

行为学

Automated Visual Cognitive Tasks for Recording Neural Activity Using a Floor Projection Maze

使用地面投影迷宫自动化视觉认知任务记录神经活动

Neuropsychological tasks used in primates to investigate mechanisms of learning and memory are typically visually guided cognitive tasks. We have ...

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结构化的康复协议改进的多功能假肢控制：一个案例研究

Advances in robotic systems have resulted in prostheses for the upper limb that can produce multifunctional movements. However, these sophisticated ...

Protocol for Data Collection and Analysis Applied to Automated Facial Expression Analysis Technology and Temporal Analysis for Sensory Evaluation

协议进行数据采集和分析应用到自动人脸表情分析技术和时序分析感官评价

We demonstrate a method for capturing emotional response to beverages and liquefied foods in a sensory evaluation laboratory using automated facial ...

Eye-Tracking Control to Assess Cognitive Functions in Patients with Amyotrophic Lateral Sclerosis

眼跟踪控制，以评估患者的认知功能与肌萎缩性脊髓侧索硬化症

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Automated, Long-term Behavioral Assay for Cognitive Functions in Multiple Genetic Models of Alzheimer's Disease, Using IntelliCage

基于 IntelliCage 的阿尔茨海默病多基因模型认知功能的自动、长期行为检测

Multiple factors&#8212;such as aging and genes&#8212;are frequently associated with cognitive decline. Genetically modified mouse models of cognitive ...

A Networked Desktop Virtual Reality Setup for Decision Science and Navigation Experiments with Multiple Participants

面向多参与者的决策科学与导航实验的网络化桌面虚拟现实设置

Investigating the interactions among multiple participants is a challenge for researchers from various disciplines, including the decision sciences and ...

Virtual Reality Experiments with Physiological Measures

用生理学方法进行虚拟现实实验

Virtual reality (VR) experiments are increasingly employed because of their internal and external validity compared to real-world observation and ...

Visual Classical Conditioning in Wood Ants

Several species of insects have become model systems for studying learning and memory formation. Although many studies focus on freely moving animals, ...

Using the Race Model Inequality to Quantify Behavioral Multisensory Integration Effects

使用种族模型不平等来量化行为多感官整合效应

Multisensory integration research investigates how the brain processes simultaneous sensory information. Research on animals (mainly cats and primates) ...