Name: ultrasound imaging-guided intracardiac injection to develop a mouse model of breast cancer brain metastases followed by longitudinal mri
Uploaded: 2014-03-06T13:45:00
Description: Watch this Scientific Journal Video about Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI at JoVE.com

我们感谢博士。黛安·帕尔梅里和NCI的帕特里夏Steeg的为我们提供的MDA-MB231Br细胞。我们感谢拉尔夫·梅森博士，杰森Reneau先生和雷蒙娜·洛佩斯女士为技术和合议支持。这项工作是由美国国防部IDEA奖W81XWH-08-1-0583和W81XWH-12-1-0317支持部分。核磁共振实验是在先进的成像研究中心，一个国家卫生研究院BTRP＃P41-RR02584设施进行，1S10RR02564801根据与VisualSonics VEVO 770进行超声引导下腔内注射。

所有动物的程序批准了得克萨斯大学西南医学中心的机构动物护理和使用委员会。 1。该MDA-MB231/Br-GFP细胞的制备<ol><li>检索和文化MDA-MB231/Br-GFP细胞在含10％FBS，1％谷氨酰胺和1％青霉素/链霉素的DMEM培养基（好心由Drs。帕尔迈和Steeg的，美国国立癌症研究所提供）。 </li><li>观察的细胞和培养基的颜色的情况下，和更换培养基每2-3天。 </li><li>胰蛋白酶消化并收集细胞，当达到80...

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在本研究中，我们已经证明，超声成像引导下左心室注射保证了精度，使每一个动物在此开发研究脑转移，无动物死亡进行了观察。影像引导下注入的妙处在于皮肤，最后进入左心室的针入度的路径可以被监控，并根据图像，这是从手动过程，需要严格的解剖标志遵循不同的调整。 脑转移瘤的颅内发展，目前的认识主要是基于对动物模型4,10组织学研究。然而，组织?...

脑转移瘤是成人最常见的颅内恶性肿瘤。预后极差，用4-6个月，即使积极治疗，中位生存期。乳腺癌脑转移1-3的高发病率的三大主要癌症之一。几个脑嗜乳腺癌线能够心内或颈动脉注射后4开发脑转移。直接注射肿瘤细胞进入左心室可以绕过肺毛细血管床，从而增加形成的脑转移，同时尽量减少内脏转移的发病率。在MDA-MB231Br线是使用最广泛的人类乳腺癌行开发脑转移在啮齿动物模型5,6中的一个。 像许多其他的研究4,7，我们已经在我们以前的研究进行心内注射的手动过程。然而，用手动获得只有50％的成功率注射小鼠的一小部分来自重复侵入性操作，如果死之前失败的试验。这里，我们引入了利用成像引导程序，以确保在注射脑寻乳腺癌细胞到无胸腺小鼠的左心室。纵向高分辨率MRI应用于遵循脑转移瘤的颅内发展。

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		<tr height="21">
			<td height="21" style="height:21px;width:413px;">DMEM</td>
			<td style="width:136px;">HyClone</td>
			<td style="width:87px;">SH30022.01</td>
			<td style="width:147px;"> </td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:413px;">Trypsin</td>
			<td style="width:136px;">Mediatech</td>
			<td style="width:87px;">25-051-C1</td>
			<td style="width:147px;"> </td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:413px;">Automatic cell counter</td>
			<td style="width:136px;">Biorad</td>
			<td style="width:87px;">TC10</td>
			<td style="width:147px;"> </td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:413px;">Isoflurane</td>
			<td style="width:136px;">Baxter International Inc.</td>
			<td style="width:87px;">1001936060</td>
			<td style="width:147px;"> </td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:413px;">VisualSonics Vevo 770 High-Resolution Imaging System </td>
			<td>Visual Sonics Inc.</td>
			<td></td>
			<td style="width:147px;"> </td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">9.4T horizontal bore magnet with a Varian INOVA Unity system </td>
			<td>Agilent</td>
			<td style="width:87px;"> </td>
			<td style="width:147px;"> </td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">O.C.T Compound</td>
			<td>Sakura Finetek USA</td>
			<td style="width:87px;">4583</td>
			<td style="width:147px;"> </td>
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ultrasound imaging-guided intracardiac injection to develop a mouse model of breast cancer brain metastases followed by longitudinal mri

乳腺癌脑转移，在IV期乳腺癌患者的30％的发生，是与高死亡率有关。中位生存期仅为6个月。关键是要具有合适的动物模型来模拟在临床情况中转移细胞的血液动力学扩散。在这里，我们介绍使用小动物超声成像引导一个准确的注射脑热带乳腺癌细胞到无胸腺裸鼠的左心室。纵向磁共振成像是用来评估颅内启动和脑转移瘤的生长。超声引导下腔内注射不仅确保了精确的注射，并在此较高的成功率也显著降低死亡率，比我们以前的手动过程。 体内高分辨率MRI高信号允许多灶性病变的可视化，小到310微米直径对T 3周注射后2加权图像。福卢W-MRI起来发现颅内肿瘤的生长和分布在整个脑转移的数量增加。

用核磁共振（在图面的分辨率78微米）的高空间分辨率，高信号病变可以被确定为小，中直径310微米（ 图2）。因为在这项研究中的转移是非常小的，坏死和水肿的发展是最小的，对T 2加权图像上的高信号病变真正表示的肿瘤块。 纵向MRI研究允许在肿瘤生长的活体无创评估。 如图3所示 ，高分辨率的MRI却能3周后，心内注射（ <stro...

Watch this Scientific Journal Video about Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI at JoVE.com

Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI

乳腺癌脑转移瘤小鼠模型的建立与超声成像引导下腔内注射MDA-MB231/Br-GFP细胞。多灶性脑转移的发展已经采用高分辨率9.4ŤMRI检查被监控纵。

超声成像引导下腔内注射到患乳腺癌脑转移其次是纵向的MRI的小鼠模型

Breast cancer brain metastasis, occurring in 30% of breast cancer patients at stage IV, is associated with high mortality. The median survival is only 6 ...

Research

JoVE Journal

Medicine

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model

在小鼠模型体内生物发光成像乳腺癌脑转移的肿瘤乏氧动力学

It is well recognized that tumor hypoxia plays an important role in promoting malignant progression and affecting therapeutic response negatively. There ...

科研

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Combination Radiotherapy in an Orthotopic Mouse Brain Tumor Model

在原位小鼠脑肿瘤模型结合放疗

Glioblastoma multiforme (GBM) are the most common and aggressive adult primary brain tumors1. In recent years there has been substantial progress in the ...

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

MRI引导下的破坏血脑屏障在大鼠模型经颅聚焦超声

Focused ultrasound (FUS) disruption of the blood-brain barrier (BBB) is an increasingly investigated technique for circumventing the BBB1-5. The BBB is a ...

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

利用磁共振成像，容积电脑断层扫描及超音波的裸鼠乳腺癌骨转移大鼠模型血管生成的多模态成像

Angiogenesis is an essential feature of cancer growth and metastasis formation. In bone metastasis, angiogenic factors are pivotal for tumor cell ...

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

乳腺癌的一种新的转基因小鼠模型：转移性乳腺癌通过瞄准导管上皮与腺病毒Cre重组酶的启动

Breast cancer is a heterogeneous disease involving complex cellular interactions between the developing tumor and immune system, eventually resulting in ...

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional 3D Model

乳腺癌细胞侵袭定量使用三维（3D）模型

It is now well known that the cellular and tissue microenvironment are critical regulators influencing tumor initiation and progression. Moreover, the ...

Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging

急性脑外伤小鼠其次是纵向双光子成像

Although acute brain trauma often results from head damage in different accidents and affects a substantial fraction of the population, there is no ...

A Preclinical Murine Model of Hepatic Metastases

肝转移的临床前小鼠模型

Numerous murine models have been developed to study human cancers and advance the understanding of cancer treatment and development. Here, a preclinical, ...