Name: in situ compressive loading and correlative noninvasive imaging of the bone-periodontal ligament-tooth fibrous joint
Uploaded: 2014-03-07T13:45:00
Description: Watch this Scientific Journal Video about In situ Compressive Loading and Correlative Noninvasive Imaging of the Bone-periodontal Ligament-tooth Fibrous Joint at JoVE.com

作者承认资助NIH / NIDCR R00DE018212（SPH），NIH/NIDCR-R01DE022032（SPH），美国国立卫生研究院/ NIDCR T32 DE07306（AJ，JDL），美国国立卫生研究院/ NCRR S10RR026645，（SPH），以及预防和恢复牙科学系和颜面部科学，加州大学旧金山分校。此外，作者承认Xradia公司研究生奖学金（AJ），Xradia公司公司，加利福尼亚州Pleasanton。 作者感谢凯瑟琳枫，加州大学旧金山分校博士为她与数据后处理援助;博士。斯蒂芬·韦纳和吉利Naveh，科学，雷霍沃特，以色列魏兹曼研究所;罗恩沙哈尔博士，耶路撒冷希伯来大学，以色列针对其特定的原位装载设备有见地的讨论。作者还要感谢生物材料与生物工程微型电脑成像设备在加州大学旧金山分校的使用微XCT和原位装载设备。

动物住房和安乐死：在本演示中使用的所有动物均按照该机构动物护理和使用委员会（IACUC）和美国国立卫生研究院（NIH）的指引，安置无病原体条件下。 提供动物与标准硬颗粒大鼠饲料和水的随机应变 。通过二氧化碳窒息，双侧开胸的两步法按照UCSF的标准协议安乐死动物经IACUC。 24小时动物献祭的范围内进行生物力学测试，以避免组织退化。 1。...

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在建立这个协议的第一个步骤涉及评估的装载架的使用刚性体的刚性。根据结果​​，刚度是显著更高使得能够使用的装载装置的标本与显著较低的硬度值进一步测试。第二步强调了仪器的使用两个阶段的装载 - 卸载曲线通过使用刚性体，不同的交联密度的PDMS材料和纤维连接生成区分不同硬度值的能力。从在间隙相的加载阶段和回送的刚度被用来确定该材料的装载和材料的回收的阻力以下卸载（...

几个实验方法继续被用来研究diarthrodial和纤维状关节生物力学。具体到牙齿器官生物力学的方法包括使用应变计1-3，光弹性方法4，5，云纹干涉6，7，电子散斑干涉8，和数字图像相关（DIC）9-14。在这项研究中，创新的方法包括非侵入性的成像使用的X射线暴露的纤维接头的内部结构（矿化组织和它们的接口由较软的区域，和接口组织如韧带）在负载相当于在体内条件。耦合到微透视显微镜原位装载装置将被使用。负载时间和负载 - 位移曲线将被收集感兴趣的新鲜收获的大鼠半侧下颌骨内装载的摩尔。该米在本研究中提出的方法的AIN的目标是通过在比较条件下，以强调牙骨的三维形貌的影响：1）无负载时加载，并且当2）同心和偏心加载。省去了切标本，并在潮湿条件下的整体完好的器官进行实验将允许三维应力状态的最大保护。这将打开调查，在各种载荷情况下理解复杂的动态过程的一个新领域。 在这项研究中，方法为只SD大鼠一个完整的纤维关节内测试PDL生物力学，被视为最佳的生物工程模型系统联合进行详细说明。实验将包括以突出关节的三个重要特点，因为它们涉及到器官水平的生物力学仿真水合条件下咀嚼负荷。这三点将包括：1）反动力与位移：肺泡插座内齿的位移和其装载反动响应，2）的三维（3D）空间构型和形态测量：用肺泡插座牙齿的几何关系，以及3）在读出器1和2中，由于在一个变化的变化加载轴， 即从同心偏心负荷。所提出的技术的三个基本读数可以应用于调查接头的脊椎动物中任一所述自适应性质变化引起的功能性要求，和/或疾病。可用于改变上述读数，特别是在不同的加载速率反动负载之间的相关性与位移，并导致反动的加载时间和载荷 - 位移曲线，突出联合生物力学整体变化。提出的协议的有效性可以通过耦合力学测试读数为3D形态测量和关节的生物力学整体评估。

<table border="0" cellpadding="0" cellspacing="0" width="652">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Bard Parker Blade</td>
			<td style="width:101px;">BD</td>
			<td style="width:123px;">MEDC-001054</td>
			<td style="width:259px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">AFM metal disk</td>
			<td style="width:101px;">Ted Pella</td>
			<td style="width:123px;">16218</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Polymethyl methacrylate&#160;</td>
			<td style="width:101px;">GC America</td>
			<td style="width:123px;">N/A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Uni-Etch</td>
			<td style="width:101px;">Bisco</td>
			<td style="width:123px;">E5502EBM</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Optibond Solo Plus</td>
			<td style="width:101px;">Kerr Corp</td>
			<td style="width:123px;">N/A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Filtek Flow</td>
			<td style="width:101px;">3M</td>
			<td style="width:123px;">N/A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Hurculite Ultra</td>
			<td style="width:101px;">Kerr</td>
			<td style="width:123px;">34346</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Tris buffer</td>
			<td style="width:101px;">Mediatech Inc.</td>
			<td style="width:123px;">N/A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Articulating paper</td>
			<td style="width:101px;"></td>
			<td style="width:123px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:169px;">Phosphotungstic Acid</td>
			<td style="width:101px;">Sigma Aldrich</td>
			<td style="width:123px;">HT152</td>
			<td></td>
		</tr>
	</tbody>
</table>

in situ compressive loading and correlative noninvasive imaging of the bone-periodontal ligament-tooth fibrous joint

这项研究表明，一种新型的生物力学测试协议。此协议的优点包括在耦合到高分辨率X-射线显微镜原位装载装置使用的，从而使模拟生理负荷和潮湿条件下的内部结构元件的可视化。实验样品将包括完整的骨牙周膜（PDL）齿纤维连接。结果将说明该协议的三个重要特点，因为它们可以应用到器官水平的生物力学：1）反动力与位移：位移牙牙槽窝内，其装载，2），三维（3D）空间配置反动响应和形态测量：用肺泡插座牙齿的几何关系，以及3）在读出器1和2中，由于在加载轴的变化， 即从同心偏心载荷的变化。提出的协议的有效性可以通过机械耦合TE进行评估蜇读数为3D形态测量和关节的生物力学整体。此外，这种技术将强调需要平衡的实验条件下，前获取纤维连接的断层图像特别反动负载。应当注意，该协议被限制为检测离体条件下的试样，并且使用造影剂进行可视化的软组织机械响应可能导致对组织和器官水平的生物力学错误的结论。

的装载装置“齿隙”恒定负荷下，“推回”，刚度，和系统漂移估计 差：循环加载和卸载部分之间，存在3秒的暂停期间，反向齿轮电机内卸真正开始前， 即作为试样拉离顶下颚（ 图3）。这一时期被称为系统，它代表一个时间段，当系统试图从闭到开的夹爪切换的反弹。但应注意的是，所有的负载周期都将包含一个类似的齿隙?...

Watch this Scientific Journal Video about In situ Compressive Loading and Correlative Noninvasive Imaging of the Bone-periodontal Ligament-tooth Fibrous Joint at JoVE.com

In situ Compressive Loading and Correlative Noninvasive Imaging of the Bone-periodontal Ligament-tooth Fibrous Joint

在这项研究中， 在加上微X射线计算机断层摄影术为纤维状关节生物力学原位装载装置使用一个将被讨论。识别与关节生物力学的整体改变的实验读数将包括：1）反动力与位移，在牙槽窝内，即牙齿位移及其装载反动响应，2）三维（3D）空间构型和形态测量， 即几何齿与齿槽插座，和3）中读出1和2的变化是由于在加载轴， 即同心或偏心载荷的变化关系。

原位压极限和骨牙周韧带齿纤维联合的相关性无创成像

This study demonstrates a novel biomechanics testing protocol. The advantage of this protocol includes the use of an in situ loading device coupled to a ...

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Bioengineering

In situ Compressive Loading and Correlative Noninvasive Imaging of the Bone-periodontal Ligament-tooth Fibrous Joint

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

高分辨率光纤内窥镜为原位细胞成像

Many biological and clinical studies require the longitudinal study and analysis of morphology and function with cellular level resolution.  ...

科研

生物工程

Demonstrating the Uses of the Novel Gravitational Force Spectrometer to Stretch and Measure Fibrous Proteins

展示新型引力光谱仪的使用拉伸和测量纤维蛋白

The study of macromolecular structure has become critical to the elucidation of molecular mechanisms and function. There are several limited, but ...

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

在体外合成的原住民，纤维长间距“和”分段长间距胶原蛋白

Collagen fibrils are present in the  extracellular matrix of animal tissue to provide structural scaffolding and  mechanical strength. These native ...

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

组织工程生物反应器的双轴机械装载设计

We  designed a loading device that is capable of applying uniaxial or biaxial  mechanical strain to a tissue engineered biocomposites fabricated for  ...

FIBS-enabled Noninvasive Metabolic Profiling

FIBS功能的无创性代谢轮廓

In the era of computational biology, new high throughput experimental systems are necessary in order to populate and refine models so that they can be ...

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

研究表皮生长因子受体的完整细胞使用相关显微镜的化学计量

This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative fluorescence ...

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of human pathology tissue using ...

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

表征细胞迁移在三维体外伤口环境

Currently, most in vitro models of wound healing, such as well-established scratch assays, involve studying cell migration and wound closure on ...

Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement

Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement

用原位滞后测量进行磁畴成像的优化设置和协议

This paper elaborates the sample preparation protocols required to obtain optimal domain patterns using the Bitter method, focusing on the extra steps ...

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

纳米传感器检测体内蛋白酶活性的无创诊断

Proteases are multi-functional enzymes that specialize in the hydrolysis of peptide-bonds and control broad biological processes including homeostasis and ...