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DOI :
10.3791/51149-v
February 5th, 2014
Chapters
0:05
Title
1:48
Preparation of Whole Cell Lysates from Cell Culture
4:00
Sample Preparation and Electrophoresis
5:50
Protein Transfer Using the iBlot Dry Blotting System
7:08
Infrared Fluorescent Protein Detection
9:14
Results: Improved Western Blot Sensitivity, Quality, and Accuracy
11:13
Conclusion
このプロトコルは、ウエスタンブロット分析を行う上での最新の進歩を探る。これらの新規な修飾は、35分間の電気泳動の実行時間を7分乾燥ブロッティング転送システムのBis-Trisゲルシステムを採用して、高解像度、品質、感度、およびウエスタンのデータの精度向上を発生する赤外蛍光タンパク質検出およびイメージング。
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