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DOI :
10.3791/51151-v
•
9:27 min
January 17th, 2014
Chapters
0:05
Title
1:34
Creating the Recombinant Vector
2:48
Recombinant Virus Generation
5:26
Plaque Assay: NO Selection
5:59
Purity of Viral Stocks: PCR Screening of Genomic DNA
7:23
Results: Recombinant Vectors and the VACV Genome
8:43
Conclusion
此处描述了一种快速和模块化的协议,用于生成重组疫苗病毒,同时使用瞬时主导选择的方法表达荧光标记的蛋白质。
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