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DOI :
10.3791/51153-v
•
9:22 min
November 26th, 2013
Chapters
0:05
Title
1:33
Creation of Artificial Transcription Factors (ATFs)
2:28
Creation of ATF Plasmid Reporter
3:57
Quantify the Effect of ATF Induction on Gene Expression Using GFP Reporter
5:33
Quantify the Effect of the ATF Induction on Cell Growth
6:42
Create Inducible Genomic Alleles
7:20
Results: Analysis of GFP Induction by ATF-promoter Pairs
8:39
Conclusion
本协议描述了实验过程的人工转录因子(申请进度查询设施)与绿色荧光蛋白同源记者和通过流式细胞仪,刺激GFP表达的申请进度查询设施能力量化的快速建设。
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