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DOI :
10.3791/51172-v
January 22nd, 2014
Chapters
0:05
Title
1:24
Reverse Transcription
3:22
Real-time PCR Amplification
5:20
Real-time PCR Data Analysis
6:24
Inter-plate Data Preparation and Analysis
8:18
Results: miRNA Expression Profiles from Cerebrospinal Fluid
9:05
Conclusion
私たちは、脳脊髄液(CSF)中のマイクロRNAをプロファイリングするリアルタイムPCRのプロトコルを記述します。 RNA抽出プロトコールを除いて、手順は他の体液、培養細胞、または組織試料から抽出したRNAに拡張することができる。
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