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DOI :
10.3791/51177-v
•
16:21 min
March 10th, 2014
Chapters
0:05
Title
1:32
Cell Slide Preparation
4:00
Preparing Microscopy Data Acquisition
6:43
Acquiring Photo-activated Localization Microscopy (PALM) Movies
8:16
PALM Movie Analysis
12:48
Representative Results: Pol1-DNA Binding in Single Bacterial Cells
15:53
Conclusion
光敏定位显微镜(PALM)结合单分子跟踪允许直接观察活的大肠杆菌细胞蛋白-DNA相互作用的定性和定量。
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