Name: trace fear conditioning in mice
Uploaded: 2014-03-20T18:45:00
Description: Watch this Scientific Journal Video about Trace Fear Conditioning in Mice at JoVE.com

This work is supported by a Baylor University Research Council grant and from a research grant from the Epilepsy Foundation.

De möss som användes i följande experiment genererades och inrymt vid Baylor University vid en omgivningstemperatur på 22 ° C, med en 14 timmars ljus och 10 timmar mörker (20:00 till 06:00 tim) dygnscykel. Mössen gavs ad libitum tillgång till mat och vatten. Alla förfaranden för att mössen var i överensstämmelse med National Institutes of Health riktlinjer för vård och användning av försöksdjur och djur Protokollet godkändes av Baylor University Djurvård och användning kommittén. <p cl...

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C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=MAP+kinase+translocates+into+the+nucleus+of+the+presynaptic+cell+and+is+required+for+long-term+facilitation+in+Aplysia.">MAP kinase translocates into the nucleus of the presynaptic cell and is required for long-term facilitation in Aplysia.</a> Neuron. 18, 899-912 (1997).</li><li>Crestani, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trace+fear+conditioning+involves+hippocampal+alpha5+GABA(A)+receptors.">Trace fear conditioning involves hippocampal alpha5 GABA(A) receptors.</a> Proc. Natl. Acad. Sci. 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There have been several studies that have elucidated the neural circuitry that underlies trace fear conditioning. Trace fear conditioning is believed to involve the CA1 of the hippocampus12-14. There is also evidence that the medial prefrontal cortex (mPFC) plays a large role in trace eye-blink conditioning15, and the mPFC has been found to be involved in trace fear conditioning. One study found that mPFC neurons provide sustained activity during the trace period, thus providing a structure that can...

I rädsla konditione ett neutralt stimulus (NS) paras med en aversiv obetingat stimulus (US). NS är normalt en ton och blir en betingad stimulus (CS) genom upprepade parningar med USA. CS kan då framkalla en betingad respons (CR), såsom frysning, i frånvaro av den bitter USA. En vanlig rädsla konditione protokollet är fördröjningskonditionering. I detta protokoll starten av NS och USA är sammanhängande eller med viss överlappning i stimulus-presentation. Även om fördröjnings rädsla konditionering är en av de vanligaste typerna av tids associativa conditioning, finns det flera andra typer av associativa konditionetidsmässiga arrangemang: samtidig konditione, bakåtkonditione och spåranläggningar 1. I spår rädsla konditione finns en stimulans fritt intervall mellan NS och USA i flera sekunder resulterar i ett &quot;spår&quot; period. Flera studier har rapporterat underskottsi trace rädsla konditione när neurotoxiska skador produceras i de strukturer som inmatas i hippocampus 2-5 eller när farmakologiska medel som används för att blockera receptor-funktionen i hippocampus. Lesion på hippocampus leder till underskott i spårkonditionering och kontextuell konditionering, men försämrar inte fördröjnings rädsla konditione 8. Det finns flera fördelar med att använda spår rädsla konditionering. Rädslan Konditione protokollet kan uppnås över en tre-dagars testperiod och möjliggör hippocampus-beroende minne som inte är rumsligt beroende. Trace rädsla konditionering kan användas som ett kompletterande prov till Morris vattenlabyrint, testa nya objektigenkänning, eller andra maze test i att undersöka hippocampus-beroende minne.

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trace fear conditioning in mice

I detta experiment presenterar vi en teknik för att mäta inlärning och minne. I spår rädsla konditione protokoll presenteras här finns fem ihopkopplingar mellan en neutral stimulus och ett obetingat stimulus. Det finns en 20 sek spår period som separerar varje konditione rättegång. På följande dag frysning mäts under presentationen av betingad stimulus (CS) och spårperioden. På tredje dagen finns det en 8 min test för att mäta kontextuella minnet. De representativa resultaten är från möss som presenterades med aversiva obetingat stimulus (chock) jämfört med möss som fick tonen presentationer utan obetingat stimulus. Trace rädsla konditione har framgångsrikt använts för att upptäcka subtila inlärning och minne underskott och förbättringar i möss som inte finns med andra metoder rädsla konditionering. Denna typ av rädsla konditione tros vara beroende av förbindelser mellan den mediala prefrontala cortex och hippocampus. En aktuell kontrovers är om denna metod tros vara amygdala oberoende. Därför behövs andra rädsla konditione tester för att undersöka amygdala beroende inlärning och minneseffekter, t.ex. genom fördröjnings rädsla konditionering.

För de representativa resultaten presenterar vi data från kontroll C57BL/6J vuxna möss som fick de neutrala stimulansligaspel med obetingat stimulus (chocktillstånd) jämfört med möss som fick neutralt stimulus men inte fått den obetingat stimulus (ingen chock tillstånd). Det är viktigt att köra detta tillstånd då först sätta upp detta beteende-test för att avgöra om protokollet har utförts korrekt. Data i figur 1 representerar utbildningsdag för C57BL/6J-m...

Watch this Scientific Journal Video about Trace Fear Conditioning in Mice at JoVE.com

Trace Fear Conditioning in Mice (Video) | JoVE

I följande experiment beskriver vi ett protokoll för spår rädsla konditionering i möss. Denna typ av associativt minne innefattar ett svepperioden som separerar neutrala stimulit och det obetingade stimulans.

Trace rädsla Conditioning i möss

In this experiment we present a technique to measure learning and memory. In the trace fear conditioning protocol presented here there are five pairings ...

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Behavior

Trace Fear Conditioning in Mice

Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice

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Kontextuell och cued rädsla Conditioning Test Med hjälp av en videoanalyssystem hos möss

The contextual and cued fear conditioning test is one of the behavioral tests that assesses the ability of mice to learn and remember an association ...

Disrupting Reconsolidation of Fear Memory in Humans by a Noradrenergic &#946;-Blocker

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Störa Återpackning av skräck minne hos människor genom en noradrenerga β-blockerare

The basic design used in our human fear-conditioning studies on disrupting reconsolidation includes testing over different phases across three consecutive ...

Protocol for Studying Extinction of Conditioned Fear in Naturally Cycling Female Rats

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Protokoll för att studera Extinction av kondition Rädsla i naturligt Cykla honråttor

Extinction of conditioned fear has been extensively studied in male rodents. Recently, there have been an increasing number of studies indicating that ...

Quantifying Social Motivation in Mice Using Operant Conditioning

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Kvantifiera social motivation i möss med användning av Operant Conditioning

In this protocol, social motivation is measured in mice through a pair of operant conditioning paradigms. To conduct the experiments, two-chambered ...

Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice

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Whisker-signalerade frånEyeblinks Classical Conditioning i Head fixerade möss

Eyeblink conditioning is a common paradigm for investigating the neural mechanisms underlying learning and memory. To better utilize the extensive ...

Reducing State Anxiety Using Working Memory Maintenance

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Reducera statens ångest genom att använda underhåll av arbetsminne

The purpose of this protocol is to explain how to examine the relationship between working memory processes and anxiety by combining the Sternberg Working ...

Classical Short-Delay Eyeblink Conditioning in One-Year-Old Children

Classical Short-Delay Eyeblink Conditioning in One-Year-Old Children (Video) | JoVE

Klassisk kort fördröjning Blink luftkonditionering i ett-åriga barn

Classical eyeblink conditioning (EBC) refers to the learned association between a conditioned stimulus (an auditory tone) and an unconditioned stimulus (a ...

Stress-Enhanced Fear Learning, a Robust Rodent Model of Post-Traumatic Stress Disorder

Stress-Enhanced Fear Learning, a Robust Rodent Model of Post-Traumatic Stress Disorder (Video) | JoVE

Stress-förbättrade rädsla lärande, en Robust gnagare modell av posttraumatiskt stressyndrom

Fear behaviors are important for survival, but disproportionately high levels of fear can increase the vulnerability for developing psychiatric disorders ...

Visual Classical Conditioning in Wood Ants

Visual Classical Conditioning in Wood Ants (Video) | JoVE

Visuella klassisk betingning i trä myror

Several species of insects have become model systems for studying learning and memory formation. Although many studies focus on freely moving animals, ...

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats (Video) | JoVE

Rädsla Inkubation Med hjälp av en utökad Fear-Conditioning Protokoll för råttor

Emotional memory has been primarily studied with fear-conditioning paradigms. Fear conditioning is a form of learning through which individuals learn the ...