Name: in vivo electrophysiological measurements on mouse sciatic nerves
Uploaded: 2014-04-13T14:00:00
Description: Watch this Scientific Journal Video about In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves at JoVE.com

这项工作是由SFB 604，东风集团MO 1421/2-1和Krebshilfe 107089（陛下）的支持。 AS是从儿童肿瘤基金会（美国纽约）收件人的青年研究者奖。

本研究根据保护的德意志联邦共和国（Tierschutzgesetz DER德意志联邦共和国）的动物法进行，并批准了图林根州厅食品安全和消费者保护（图林根森Landesamt献给Lebensmittelsicherheit UND Verbraucherschutz）。 1，设置的测量<ol><li>麻醉小鼠通过异氟烷/ O 2吸入-对麻醉3％的起始，在100％的氧气维持2％异氟醚（ 图1）。通过测试简单的反射，如运动反射和低档疼痛的敏?...

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所描述的协议提供了一个简单而可靠的方法，而不需要暴露感兴趣的神经，以确定麻醉小鼠坐骨神经传导性能。然而，这一实验过程由穿刺针引起组织损伤。因此，这是一个合理的选择来完成录音后牺牲动物。然而，相对于其他更具侵入性的程序，这需要神经前录音曝光，组织损伤相对较小3,14。因此，重复的测量是可能的，并且取决于相应的研究设计。然而，某些点具有以保证一致的结?...

电生理测量是必不可少的工具在临床和实验室环境调查周围神经功能的完整性。在人类中，有大量神经肌肉疾病和神经病变的诊断依赖于电生理学测量。通过测量神经性质传导速度或信号的电势的振幅，能够表征外周神经疾病的大致原点。 神经传导速度是高度依赖于由髓鞘启用快速信号传播。因此，脱髓鞘过程一般表现出下降传导速度4。复合肌肉动作电位（CMAP） -与功能性轴突的数量关联-是轴索损害时显著减少5的指标。 因此，通过电生理学方法手段的外周神经损伤的病因能够被辨别，如遗传性神经病6,7，糖尿病性神经病8,9，慢性炎症性脱髓鞘性多神经病（CIDP）10，或代谢性神经病11。 通常，在人类应用上的腓肠或尺神经的非侵入性的录音是优选的。在小鼠中，它是直接分析坐骨神经的神经性，同时含有较大的外周神经系统（PNS）中最大的神经 - 及肌肉运动和感觉系统的小口径轴突。 因为这里展示的过程是一个快速，简单和可靠的方法来测量相关的电生理学上的完整的小鼠外周神经的所有标准值。通过采取录音从保存生物，神经环境的生理条件有保证。

<table border="0" cellpadding="0" cellspacing="0" width="917">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:321px;">Concentric Needle Electrodes (Stimulation)</td>
			<td style="width:311px;">Natus Medical Incorporated 
			San Carlos, CA 94070, USA</td>
			<td style="width:119px;">9013S0901</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Digital Ring Electrodes (Recording)</td>
			<td style="width:311px;">Natus Medical Incorporated 
			San Carlos, CA 94070, USA</td>
			<td>9013S0302</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">ToM - Tower of Measurement (A/D converter)</td>
			<td>GJB Datentechnik GmbH, Langewiesen, Germany</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">AtisaPro, Data acquisition &#38; analysis software</td>
			<td>GJB Datentechnik GmbH, Langewiesen, Germany</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">HSE-Stimulator T</td>
			<td colspan="2">Hugo Sachs Elektronik, Hugstetten, Germany</td>
			<td></td>
		</tr>
	</tbody>
</table>

in vivo electrophysiological measurements on mouse sciatic nerves

电生理学研究允许各种神经肌肉疾病的合理的分类，并帮助连同神经病理学技术，在病理生理学1的理解。在这里，我们描述了在体内进行对小鼠坐骨神经的神经电生理研究的方法。 将动物用异氟烷麻醉，以确保镇痛试验小鼠并在此期间，约需30分钟/动物的测量不受干扰的工作环境。 37℃恒定的体温是由加热板维护和直肠温探头2连续测量。另外，心电图（ECG）中，以便测量被定期地记录连续监视所研究的动物的生理状态。 电生理记录均于坐骨神经，日最大神经进行ë外周神经系统（PNS），与两个肌肉运动和感觉纤维束供给鼠标后肢。在我们的协议，坐骨神经留在原位 ，并因而不具有要被提取或暴露，允许测量无任何不良神经刺激随着实际录音。使用适当的针电极3正进行近端和远端的神经刺激，登记所发送的电位与感测电极在腓肠肌。经过数据处理的，可靠的和对神经传导速度（NCV）和化合物肌肉动作电位（CMAP）高度一致的值，关键参数为外周神经机能总值的量化，可以实现的。

<html>我们进行了一系列体内对12只小鼠的坐骨神经电生理测量的总本研究：每组6只性别。的测量进行了与所提出的协议，并提供了以下的结果： 雄性和雌性小鼠显示约20米/秒，平均坐骨神经传导速度（ 图5）。这与文献中的其他测量是一致的。此外，它表明，根据我们的数据是男性和女性之间没有相关的差异神经传导速度。 <p class="jove_content" fo:keep-togeth...

Watch this Scientific Journal Video about In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves at JoVE.com

In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves

在对小鼠坐骨神经体内的电测量

体内神经传导特性的测量体现了强大的工具来表征神经肌肉疾病的多种动物模型。在这里，我们提出了一个简单而可靠的协议，其中对麻醉小鼠坐骨神经电生理分析可以执行。

Electrophysiological studies allow a rational classification of various neuromuscular diseases and are of help, together with neuropathological ...

Research

JoVE Journal

Neuroscience

In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves

Electrophysiological Measurements from a Moth Olfactory System

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科研

神经科学

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Pain is an unpleasant sensory and emotional experience. Since infants cannot verbally report their experiences, current methods of pain assessment are ...

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

使用超声波血脑屏障破坏和锰增强MRI的脑功能成像

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Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

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Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

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在体内双光子成像的经验依赖的分子在大脑皮质神经元的变化

The brain's ability to change in response to experience is essential for healthy brain function, and abnormalities in this process contribute to a variety ...

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Simultaneous electrophysiological and fluorescent imaging recording methods were used to study the role of changes of membrane potential or current in ...

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体内基因转移到雪旺细胞在啮齿动物坐骨神经电穿孔

The formation of the myelin sheath by Schwann cells (SCs) is essential for rapid conduction of nerve impulses along axons in the peripheral nervous ...

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Nerve-resident immune cells in the peripheral nervous system (PNS) are essential to maintaining neuronal integrity in a healthy nerve. The immune cells of ...

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Assessing the functionality of the nerve axon provides detailed information on the progression of neuromuscular disorders. Electrophysiological recordings ...