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DOI :
10.3791/51183-v
January 15th, 2014
Chapters
0:05
Title
1:43
Day 1 - Lysing Bacterial Cultures
3:24
Day 2 - Enzymatic Digestions
6:28
Day 3 - Preparation of Samples for UPLC
9:29
Results: UPLC Chromatograms of PG Digested from E. coli Sacculi
10:36
Conclusion
细菌细胞壁由多肽组成,多肽交叉连接的糖链大分子网络。超性能液态色谱为多肽类成分的新发现提供了高分辨率和高吞吐量。我们提出了一个隔离细胞壁(sacculi)的程序,以及随后通过 UPLC 进行分析的准备。
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