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DOI :
10.3791/51189-v
March 17th, 2014
Chapters
0:05
Title
1:56
Hydrodynamic Gene Transfer
2:54
Adoptive Transfer of Common Dendritic Progenitors (CDPs)
6:38
Isolation and Enumeration of Peyer's Patch Plasmacytoid Dendritic Cells
9:36
Results: Analysis of PP pDC in Ifnar/- Mice Upon Flt3L HGT and Adoptive Transfer of CPDs
13:12
Conclusion
このプロトコルは、FACSを介した細胞の分離、流体力学的遺伝子導入、およびパイエル板の免疫サブセットのフロー分析を含む技術を使用して、一般的な樹状細胞前駆体からのパイエル板での形質細胞様樹状細胞の分化を評価するための実験手順について説明します。
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