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DOI :
10.3791/51192-v
May 16th, 2014
Chapters
0:05
Title
1:47
Kidney Mononuclear Cell (KMNC) Isolation
3:48
DN T Cell Isolation from CD45+ KMNC
5:26
Results: Representative Kidney DN αβ T Cell Purity
6:31
Conclusion
DNabT细胞是罕见的外周T细胞之间;然而,他们是丰富的在某些非淋巴组织。从非淋巴组织中分离的DN T细胞的难度阻碍了其功能的分析尽管越来越多的认可的病理生理意义。我们描述了一种新的协议,用于从小鼠肾脏高度纯化的DN T细胞的隔离。
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