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DOI :
10.3791/51204-v
•
23:21 min
April 19th, 2014
Chapters
0:04
Title
2:48
Growing Nicotiana Plants
3:55
Vacuum Infiltration of Agrobacterium into Nicotiana Plants
7:41
Western Blot Analysis
9:40
GFP Imaging
10:21
Results: Agrobacterium tumefaciens Infiltration is an Effective Method for Transient Protein Production in Nicotiana Species
22:19
Conclusion
基于真空浸润土壤杆菌携带发射向量( 烟草花叶病毒为基础)是一种快速和经济的方法来生产疫苗抗原和治疗性蛋白质的瞬态蛋白生产中烟草属植物。我们简化了程序,并通过优化细菌培养的条件,在选择宿主物种,并同时引入的RNA沉默抑制改善目标的积累。
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