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DOI :
10.3791/51207-v
•
8:11 min
September 1st, 2015
Chapters
0:05
Title
1:24
Preparation of (Primary or MT4 CD4+) Target T Cells and (PBMC or pDC) Sensing Cells
2:56
Infected CD4+ T Cell Co-culture with Sensing Cells
4:02
Measurement of Bioactive Type-I IFN Using HEK-Blue IFN-α/β Reporter Cells
5:23
Results: Characterization and Co-culture of Freshly Isolated PBMCs and Infected MT4 T Cells
7:27
Conclusion
不像无细胞的HIV-1颗粒,感染的CD4 + T细胞是由浆细胞样树突细胞(pDC细胞)有效地感测。这个手稿描述了一种方法,其中外周血单核细胞(PBMC)或分离的pDC是共培养的HIV-1感染的T细胞的pDC评估先天感测的评估,I型干扰素的释放。
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