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DOI :
10.3791/51219-v
June 15th, 2014
Chapters
0:05
Title
1:36
hNPC Observations
2:13
Chopper Setup
3:22
Pre-chop Procedure
5:58
Chop Procedure
7:08
Post-chop Procedure
8:11
Representative Results of hNPCs Aggregates Using a cGMP-applicable Expansion Method
9:04
Conclusion
这个协议描述了一种新颖的机械斩波方法,它允许球形神经干细胞和祖细胞的聚集体的膨胀而不分离为单个细胞悬浮液。维持细胞/细胞接触可超过40代快速稳定增长。
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