Name: a murine model of cervical spinal cord injury to study post-lesional respiratory neuroplasticity
Uploaded: 2014-05-28T13:00:00
Description: Watch this Scientific Journal Video about A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity at JoVE.com

Dette arbeidet er støttet av midler fra EU sjuende rammeprogram (FP7/2007-2013) i henhold til tildelingsavtale nr 246556 (europeisk prosjekt RBUCE-UP), HandiMedEx tildelt av den franske Public Investment Board. Marcel Bonay ble støttet av den Chancellerie des Universites de Paris (Legs Poix), den Fonds de Dotation Recherche en Santé Respiratoire, og Centre d&#39;Assistance Respiratoire à Bosted d&#39;Île de France (CARDIF)

Denne protokollen ble godkjent av Etisk komité av RBUCE-UP stol of Excellence (University of Paris Sud, tilskuddsavtalen nr. 246556) og Université de Versailles Saint-Quentin-en-Yvelines. En. Utarbeidelse av Sterilisert kirurgiske instrumenter <ol><li> Rengjør kirurgiske instrumenter med laboratorie vaskemiddel. </li><li> Autoklav instrumentene før operasjonen. </li><li> I en kirurgisk sesjon, sterilisere de verktøy ved å plassere spissene i en varm kule sterilisator i 10 min ved 180...

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G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+crossed+phrenic+phenomenon:+a+model+for+plasticity+in+the+respiratory+pathways+following+spinal+cord+injury.">The crossed phrenic phenomenon: a model for plasticity in the respiratory pathways following spinal cord injury.</a> J Appl Physiol. 94, 795-810 (2003).</li><li>Vinit, S., Gauthier, P., Stamegna, J. C., Kastner, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+cervical+lateral+spinal+cord+injury+results+in+long-term+ipsilateral+hemidiaphragm+paralysis.">High cervical lateral spinal cord injury results in long-term ipsilateral hemidiaphragm paralysis.</a> J Neurotrauma. 23, 1137-1146 (2006).</li><li>Fuller, D. D., Johnson, S. M., Johnson, R. A., Mitchell, G. 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Tekniske problemer med å gjøre C2 Injury Model C2 skade murine modellen er et interessant verktøy for å studere luftpost lesional nevroplastisitet. Imidlertid er fremgangsmåten for å produsere en reproduserbar og pålitelig modell er mange og hver av dem vil kunne påvirke resultatet av undersøkelsen. For eksempel under intubasjon prosessen, er ekstrem forsiktighet for å bli tatt ettersom orotracheal røret kan produsere en betennelse i luftrøret, noe som kan føre ...

Ryggmargstraumer er en vanlig skade observeres i den menneskelige populasjonen med dramatiske forekomst, slik som permanent lammelse. Imidlertid, alvorligheten av skaden er avhengig av nivået og omfanget av den opprinnelige traumer. Respirasjonssvikt er den ledende årsaken til dødelighet følgende øvre cervical ryggmargsskade (SCI) en. For tiden, er den eneste terapeutiske behandlingen for å plassere pasienten i henhold til ventilasjonshjelp. Etter noen få pasienter kan avvent av ventilasjonshjelp 2, på grunn av spontan gjenvinning som oppstår med post-lesional forsinkelse, er det behov for å utvikle nye, innovative ikke-invasive terapeutika presser 3. Å ha en god standardisert preklinisk modell for å undersøke effekten av en cervikal SCI på respiratorisk insuffisiens, og derfor, for å studere anvendelsen av antatte terapeutiske strategier, er viktig. I denne tekniske artikkelen beskriver vi en bestemt pre-klinisk murine modell of respiratorisk svekkelse forårsaket av en delvis cervical SCI på C2-nivå. Denne modellen er i dag brukt av flere laboratorier rundt om i verden (for anmeldelser: 4-13). Imidlertid kan observeres små forskjeller i den kirurgiske prosedyren mellom de forskjellige undersøkere for å generere denne spesielle cervical skade murine modellen. Effekten av en C2 SCI på luftutgangs ble først beskrevet i 1895 av Porter 14. En cervical hemisection induserer en deafferentation av phrenic motoneurons fra sin sentrale stasjonen (ligger i rVRG i hjernestammen, figur 1A) på ipsilaterale side av skade, som fører til en stille phrenic nerve aktivitet og den påfølgende membran lammelser. Den kontralaterale side forblir intakt og tillater dyret å overleve. I motsetning til forskjellige SCI plassert i en nedre rygg segment (for eksempel en contusive skade på C4 nivå 15), blir integriteten av phrenic motoneuron kjernen på begge sider bevart. Etter en cervical C2 skade, kan noen spontan aktivitet observeres på ipsilaterale side (phrenic og membran) på grunn av en aktivering av kontralaterale tause synaptiske trasé som krysset rygglinjen på segmentnivå C3-C6 (Crossed phrenic trasé, CPP, figur 1B) . Aktiveringen av CPP, som er, per definisjon, en C2 hemisection kombinert med en kontralateral phrenicotomy som induserer en ipsilaterale delvis phrenic nerve utvinning, kan oppstå fra timer til uker etter skade 16-18. Den virkelige fordelaktige effekten av dette CPP pathway på luft utvinning er begrenset 19 og videre undersøkelser, og behandlingen bør bli utviklet for å forbedre graden av spontan restaurering 3.. Denne protokollen gir en kraftig type pre-klinisk murine modell for å studere luftpost lesional plastisitet på ulike nivåer (åndedretts fysiologi fra før og phrenic motoneurons, interneurons, molekylær og mobilnettetr, bevegelse av den fremre ekstremiteten for eksempel), så vel som en modell for å teste invasive og ikke-invasive terapeutiske strategier rettet for å forbedre luftveis og lokomotorisk restitusjon etter C2 partialt cervical ryggmargsskade.

<table border="0" cellpadding="0" cellspacing="0" width="573">
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	<tbody>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;width:572px;">Animal</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Male Sprague Dawley Rat</td>
			<td>Janvier</td>
			<td></td>
			<td>225-250g</td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Surgical Instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Student Dumont #5 forceps</td>
			<td>Fine Science Tool</td>
			<td>91150-20</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Student Standard Pattern Forceps</td>
			<td>Fine Science Tool</td>
			<td>91100-12</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Mayo-Stille Scissors</td>
			<td>Fine Science Tool</td>
			<td>14013-15</td>
			<td>Curved</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Student Vannas Spring Scissors</td>
			<td>Fine Science Tool</td>
			<td>91500-09</td>
			<td>Straight</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Spring Scissors - 8 mm Blades</td>
			<td>Fine Science Tool</td>
			<td>15025-10</td>
			<td>Straight Blunt/Blunt</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Friedman Pearson Rongeur</td>
			<td>Fine Science Tool</td>
			<td>16121-14</td>
			<td>Curved</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dissecting Knife - Fine Tip</td>
			<td>Fine Science Tool</td>
			<td>10055-12</td>
			<td>Straight</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Olsen-Hegar Needle Holder</td>
			<td>Fine Science Tool</td>
			<td>12002-14</td>
			<td>Serrated</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Weitlaner-Locktite Retractor</td>
			<td>Fine Science Tool</td>
			<td>17012-11</td>
			<td>2x3 Blunt</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Absorbable surgical sutures</td>
			<td>Centravet</td>
			<td>BYO001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Equipment</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hot Bead Steriliser</td>
			<td>Fine Science Tool</td>
			<td>18000-45</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Catheter&#160;</td>
			<td>Centravet</td>
			<td>CAT188</td>
			<td>16 gauge</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Laryngoscope</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Guide wire</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Laryngeal mirror</td>
			<td>Centravet</td>
			<td>MIR011</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lactated Ringers</td>
			<td>Centravet</td>
			<td>RIN020</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Syringe</td>
			<td>Centravet</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Needle</td>
			<td>Centravet</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">O2</td>
			<td>Air Liquid</td>
			<td>I1001M20R2A001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">683 RodentT Ventilator 115/230V</td>
			<td>Havard Apparatus</td>
			<td>55-0000</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Stand-Alone Vaporizer</td>
			<td>WPI</td>
			<td>EZ-155</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Thin line heated bed</td>
			<td>WPI</td>
			<td>EZ-211</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Air canister</td>
			<td>WPI</td>
			<td>EZ-258</td>
			<td></td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Drugs</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Carprofen</td>
			<td>Centravet</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Rimadyl</td>
			<td>Centravet</td>
			<td>RIM011</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Buprenorphine</td>
			<td>Centravet</td>
			<td>BUP001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Baytril</td>
			<td>Centravet</td>
			<td>BAY001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dexmedetomidine</td>
			<td>Centravet</td>
			<td>DEX010</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Atipamezole</td>
			<td>Centravet</td>
			<td>ANT201</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Betadine Solution</td>
			<td>Centravet</td>
			<td>VET002</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Isoflurane</td>
			<td>Centravet</td>
			<td>VET066</td>
			<td></td>
		</tr>
	</tbody>
</table>

a murine model of cervical spinal cord injury to study post-lesional respiratory neuroplasticity

En cervical ryggmargsskade induserer permanent lammelse, og ofte fører til åndenød. Til dags dato har ingen effektive behandlingsformer er utviklet for å forbedre / forbedre den respirasjonssvikt følgende høy cervical ryggmargsskade (SCI). Her foreslår vi en murine pre-klinisk modell av høy SCI ved livmorhals 2 (K2) minimal metamerisme nivå for å studere diverse post-lesional åndedretts nevroplastisitet. Teknikken består av en kirurgisk delvis skade på C2-nivå, noe som vil fremkalle en hemiparalysis av membranen på grunn av en deafferentation av phrenic motoneurons fra luft sentre lokalisert i hjernestammen. Den kontralaterale side av skaden forblir intakt og tillater dyret utvinning. I motsetning til andre SCIS som påvirker bevegelsesfunksjonen (på thorax og lumbale nivå), vil ikke lungefunksjon krever ikke dyr motivasjon og kvantifisering av underskuddet / utvinning kan lett utføres (diafragma og phrenic nerve opptaks, hele kroppen ventilasjon). Denne pre-klinisk C2 SCI-modellen er en kraftig, nyttig og pålitelig pre-klinisk modell for å studere ulike luftveis og ikke-respiratoriske Neuroplasticity hendelser på ulike nivåer (molekylære til fysiologi) og å teste ulike antatte terapeutiske strategier som kan forbedre åndedrett i SCI pasienter.

Skadene Suksessen og reproduserbarheten av denne bestemt eksperimentell modell, er avhengig av erfaringen til hver manipulator / kirurg. Den påfølgende mengde av respiratorisk utvinning (phrenic nerve-aktivitet og membranaktivitet) etter en C2 skade er korrelert med den gjenværende ventrolateral spart hvit substans 21.. Siden skaden er &quot;håndlaget» og krever litt øvelse fra kirurgen, har omfanget av hver skade for å bli kontrollert av histologiske tekni...

Watch this Scientific Journal Video about A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity at JoVE.com

A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity

En Murint Model of Cervical Spinal Cord Injury å studere Post-lesional Respiratory Neuroplasticity

Respirasjonssvikt er den ledende dødsårsaken etter en cervical ryggmargsskade. Å ha en reproduserbar, kvantifiserbar, og pålitelig pre-kliniske dyremodell av respiratorisk svikt forårsaket av en delvis cervical skade vil hjelpe til å forstå den etterfølgende luftveiene og ikke-respiratoriske neuroplasticity og tillater å teste mulige reparasjons strategier.

A cervical spinal cord injury induces permanent paralysis, and often leads to respiratory distress. To date, no efficient therapeutics have been developed ...

Research

JoVE Journal

Medicine

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury

Respiratory compromise due to phrenic motor neuron loss is a debilitating consequence of a large proportion of human traumatic spinal cord injury (SCI) ...

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

Akutt og kronisk Taktil Sensory Testing etter ryggmargsskade i Rats

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor

En Contusive Modell av Unilateral Cervical ryggmargsskade Bruke Infinite Horizon impactor

While the majority of human spinal cord injuries occur in the cervical spinal cord, the vast majority of laboratory research employs animal models of ...

Controlled Cervical Laceration Injury in Mice

Kontrollert Cervical Laserasjon Injury in Mus

Use of genetically modified mice enhances our  understanding of molecular mechanisms underlying several neurological disorders  such as a spinal cord ...

A Contusion Model of Severe Spinal Cord Injury in Rats

En Contusion modell av alvorlig ryggmargsskade i rotter

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment (RMCA) in Individuals with Chronic Spinal Cord Injury

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment RMCA in Individuals with Chronic Spinal Cord Injury

Evaluering av Respiratory Muscle Activation hjelp Respiratory motorstyrekort Assessment (RMCA) hos personer med kronisk ryggmargsskade

During breathing, activation of respiratory muscles is coordinated by integrated input from the brain, brainstem, and spinal cord. When this coordination ...

Controlled Cortical Impact Model for Traumatic Brain Injury

Kontrollert Cortical Impact Modell for traumatisk hjerneskade

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

En Radio-telemetrisk system for å overvåke kardiovaskulær funksjon i rotter med ryggmargstran og Embryonic Neural Stem Cell Grafts

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

Synergisk Bruk av Neural forløperceller og Selv montering Peptider i Experimental Cervical ryggmargsskade

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

Calibrated Forceps Model of Spinal Cord Compression Injury

Calibrated pins Modell av ryggmargskompresjon Injury

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...