Name: a murine model of cervical spinal cord injury to study post-lesional respiratory neuroplasticity
Uploaded: 2014-05-28T13:00:00
Description: Watch this Scientific Journal Video about A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity at JoVE.com

Bu çalışma hibe sözleşmesi No 246556 (Avrupa projesi RBUCE-UP) kapsamında Avrupa Birliği Yedinci Çerçeve Programı (FP7/2007-2013) fon tarafından desteklenen, HandiMedEx Fransız Kamu Yatırım Kurulu tarafından tahsis. Marcel Bonay Chancellerie des Üniversiteler de Paris (Bacaklar Poix), Sante Respiratoire tr Fonds de Dotation Recherche ve Centre d&#39;Yardımlaşma Respiratoire bir ikametgah d&#39;Île de France (Cardif) tarafından desteklenen

Bu protokol Excellence (Paris Sud, hibe sözleşmesi No 246556 Üniversitesi) ve Université de Versailles Saint-Quentin-en-Yvelines RBUCE-UP sandalyenin Etik komitesi tarafından onaylandı. Steril Cerrahi Aletler 1. Hazırlanması <ol><li> Laboratuvar deterjan ile cerrahi aletleri temizleyin. </li><li> Ameliyat öncesi araçları otoklavlayın. </li><li> Cerrahi bir oturumda, 2 ameliyatları arasında 180 ° C&#39;de 10 dakika boyunca sıcak bir boncuk sterilizatör ipuçlarını yerleş...

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C2 Yaralanma Modeli Yapımı Teknik Sorunlar C2 yaralanma fare modeli solunum lezyon sonrası nöroplastisiteyi incelemek için ilginç bir araçtır. Ancak, tekrar üretilebilir ve güvenilir bir modeli üretmek için gerekli adımlar çok sayıda ve her biri çalışmanın sonuçları etkileyebilecek. Örneğin, entübasyon işlemi sırasında, aşırı bakım orotrakeal tüp gibi yaralanma nedeniyle kendisine başlangıç ​​solunum yetersizliği yanında obstrüktif s...

Spinal kord travması gibi kalıcı felç gibi dramatik olaylar ile insan nüfusunun gözlenen yaygın bir yaralanma olduğunu. Bununla birlikte, yaralanma şiddetine başlangıç ​​travma seviyesi ile boyutuna bağlıdır. Solunum yetmezliği üst servikal spinal kord yaralanması (SKY) 1 Aşağıdaki mortalitenin önde gelen nedenidir. Şu anda, tek terapötik tedavi ventilasyon yardımı altında hasta yerleştirmektir. Birkaç hastada lezyon sonrası gecikme oluşur spontan iyileşme, solunum yardımı 2 kapalı sütten olabilir bu yana, yenilikçi non-invaziv ilaçlar geliştirebilmek için ihtiyaç acil 3&#39;tür. Farazi tedavi stratejilerinin uygulanmasını incelemek için, bu nedenle solunum yetmezliği üzerinde servikal SCI etkisini araştırmak ve iyi bir standart klinik öncesi modeli olması esastır. Bu teknik makalede, biz belirli bir klinik öncesi fare modeli o tariff solunum bozukluğu C2 seviyesinde kısmi servikal SCI tarafından uyarılan. Bu model şu anda (: 4-13 değerlendirmeleri için) dünyanın çeşitli laboratuvarlar tarafından kullanılır. Ancak, cerrahi prosedür hafif farklılıklar bu özel servikal yaralanma fare modeli oluşturmak için farklı araştırmacılar arasında görülebilir. Solunum çıkışında C2 SCI etkisi ilk Porter 14 tarafından 1895 yılında tarif edilmiştir. Servikal hemiseksiyona sessiz frenik sinir aktivitesi ve sonraki diyafram felç yol açan, yaralanma aynı taraftaki (beyin sapı, Şekil 1A rVRG bulunan), merkez sürücüden frenik motor nöronlar bir deaferentasyon neden olur. Karşı taraf bozulmadan kalır ve hayvan hayatta kalmak için izin verir. Bir düşük değerli spinal kesimi (C4 düzeyinde 15, örneğin bir çürük hasarı) bulunan farklı SCI farklı olarak, her iki tarafta frenik motonöron çekirdeğin bütünlüğü korunur. Bir červ sonraical C2 yaralanması, bazı spontan aktivite nedeniyle segmental seviyesi C3-C6 (Çapraz Frenik yolları, CPP, Şekil 1B) spinal orta hat geçti taraf sessiz sinaptik yolların bir aktivasyonuna (frenik ve diyafram), ipsilateral tarafta görülebilir . Olan CPP, aktivasyonu, tanımı gereği, bir taraf kısmi frenik sinir kurtarma neden bir kontralateral phrenicotomy ile birlikte bir C2 hemiseksiyona, saat hafta sonrası yaralanma 16-18 oluşabilir. Solunum kurtarma bu CPP yolun gerçek yararlı etkisi 19 sınırlıdır ve daha fazla araştırma ve tedavi spontan restorasyonu 3 büyüklüğünü artırmak için geliştirilmelidir. Bu protokol (öncesi ve frenik motor nöronlar, internöronlar, moleküler ve cellula solunum fizyolojisi çeşitli düzeylerde solunum lezyon sonrası plastisiteyi incelemek için klinik öncesi fare modelinde güçlü bir türü sağlarr, örneğin, ön bacak) lokomosyon yanı sıra C2 kısmi servikal omurilik yaralanmasının ardından solunum ve lokomotor kurtarma geliştirmek amaçlanmıştır invaziv ve non-invaziv tedavi stratejileri test etmek için bir model.

<table border="0" cellpadding="0" cellspacing="0" width="573">
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	<tbody>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;width:572px;">Animal</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Male Sprague Dawley Rat</td>
			<td>Janvier</td>
			<td></td>
			<td>225-250g</td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Surgical Instruments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Student Dumont #5 forceps</td>
			<td>Fine Science Tool</td>
			<td>91150-20</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Student Standard Pattern Forceps</td>
			<td>Fine Science Tool</td>
			<td>91100-12</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Mayo-Stille Scissors</td>
			<td>Fine Science Tool</td>
			<td>14013-15</td>
			<td>Curved</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Student Vannas Spring Scissors</td>
			<td>Fine Science Tool</td>
			<td>91500-09</td>
			<td>Straight</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Spring Scissors - 8 mm Blades</td>
			<td>Fine Science Tool</td>
			<td>15025-10</td>
			<td>Straight Blunt/Blunt</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Friedman Pearson Rongeur</td>
			<td>Fine Science Tool</td>
			<td>16121-14</td>
			<td>Curved</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dissecting Knife - Fine Tip</td>
			<td>Fine Science Tool</td>
			<td>10055-12</td>
			<td>Straight</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Olsen-Hegar Needle Holder</td>
			<td>Fine Science Tool</td>
			<td>12002-14</td>
			<td>Serrated</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Weitlaner-Locktite Retractor</td>
			<td>Fine Science Tool</td>
			<td>17012-11</td>
			<td>2x3 Blunt</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Absorbable surgical sutures</td>
			<td>Centravet</td>
			<td>BYO001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Equipment</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hot Bead Steriliser</td>
			<td>Fine Science Tool</td>
			<td>18000-45</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Catheter&#160;</td>
			<td>Centravet</td>
			<td>CAT188</td>
			<td>16 gauge</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Laryngoscope</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Guide wire</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Laryngeal mirror</td>
			<td>Centravet</td>
			<td>MIR011</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lactated Ringers</td>
			<td>Centravet</td>
			<td>RIN020</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Syringe</td>
			<td>Centravet</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Needle</td>
			<td>Centravet</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">O2</td>
			<td>Air Liquid</td>
			<td>I1001M20R2A001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">683 RodentT Ventilator 115/230V</td>
			<td>Havard Apparatus</td>
			<td>55-0000</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Stand-Alone Vaporizer</td>
			<td>WPI</td>
			<td>EZ-155</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Thin line heated bed</td>
			<td>WPI</td>
			<td>EZ-211</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Air canister</td>
			<td>WPI</td>
			<td>EZ-258</td>
			<td></td>
		</tr>
		<tr height="21">
			<td colspan="4" height="21" style="height:21px;">Drugs</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Carprofen</td>
			<td>Centravet</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Rimadyl</td>
			<td>Centravet</td>
			<td>RIM011</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Buprenorphine</td>
			<td>Centravet</td>
			<td>BUP001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Baytril</td>
			<td>Centravet</td>
			<td>BAY001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dexmedetomidine</td>
			<td>Centravet</td>
			<td>DEX010</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Atipamezole</td>
			<td>Centravet</td>
			<td>ANT201</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Betadine Solution</td>
			<td>Centravet</td>
			<td>VET002</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Isoflurane</td>
			<td>Centravet</td>
			<td>VET066</td>
			<td></td>
		</tr>
	</tbody>
</table>

a murine model of cervical spinal cord injury to study post-lesional respiratory neuroplasticity

Servikal spinal kord yaralanması kalıcı felç neden olur, ve genellikle solunum sıkıntısı yol açar. Bugüne kadar, hiçbir verimli terapötiklerin yüksek servikal spinal kord yaralanması (SKY) sonrası solunum yetmezliği iyileştirmek / geliştirmek için geliştirilmiştir. Burada çeşitli lezyon sonrası solunum nöroplastisiteyi çalışma servikal 2 (C2) metamerik düzeyde yüksek SCI bir kemirgen pre-klinik modeli öneriyoruz. Bu teknik nedeniyle beyin bulunan solunum merkezlerinden frenik motor nöronlar bir deaferentasyon üzere diyaframa bir hemipleji neden olacak C2 düzeyinde bir cerrahi kısmi yaralanma oluşur. Yaralanma karşı taraf bozulmadan kalır ve hayvan iyileşme sağlar. (Torakal ve lomber düzeyde) lokomotor fonksiyonunu etkileyen diğer omurilik aksine, solunum fonksiyon hayvan motivasyon gerektirmez ve açığı / kurtarma miktar kolayca (diyafram ve frenik sinir kayıt yapılabilirs, tüm vücut havalandırma). Bu klinik öncesi C2 SCI modeli farklı seviyelerde (fizyoloji, moleküler) çeşitli solunum ve non-solunum nöroplastisite olayları incelemek ve solunum geliştirmek olabilir çeşitli farazi tedavi stratejileri test etmek için güçlü, kullanışlı ve güvenilir klinik öncesi modeli SCI hastalar.

Yaralanma Kapsamı Başarı ve bu özel deneysel modelin uyarlık her manipülatör / cerrahın deneyimine bağlıdır. Kalan ventrolateral beyaz madde 21 bağışladı ile bir C2 yaralanma takiben solunum kurtarma (frenik sinir aktivitesi ve diyafram aktivite) sonraki miktarı ilişkilidir. Yaralanma &quot;el yapımı&quot; olduğu ve cerrah bazı pratik gerektirir yana, her yaralanma ölçüde tam boyutunu belirlemek için histolojik teknikler (paraformaldehitle...

Watch this Scientific Journal Video about A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity at JoVE.com

A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity

Solunum yetmezliği servikal omurilik yaralanması sonucu ölüm önde gelen nedenidir. Kısmi servikal yaralanma tarafından uyarılan solunum yetmezliği, tekrarlanabilir ölçülebilir ve güvenilir klinik öncesi hayvan modeli olan müteakip solunum ve non-solunum nöroplastisiteyi anlamak için yardımcı ve varsayılan onarım stratejilerini test sağlayacaktır.

Post-lezyonlu Solunum nöroplastisite Eğitim için Servikal Spinal Kord Yaralanması bir fare modeli

A cervical spinal cord injury induces permanent paralysis, and often leads to respiratory distress. To date, no efficient therapeutics have been developed ...

Research

JoVE Journal

Medicine

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury

Amyotrophic Lateral Sclerosis ve Travmatik Spinal Kord Yaralanması Servikal Ventral Horn Hedefleme intraspinal Hücre Transplantasyonu

Respiratory compromise due to phrenic motor neuron loss is a debilitating consequence of a large proportion of human traumatic spinal cord injury (SCI) ...

Tıp

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

Sıçanlarda Spinal Kord Yaralanma sonrası Akut ve Kronik Dokunsal Duyu Testi

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor

Sonsuz Horizon Darbeli kullanarak tek taraflı Servikal Spinal Kord Yaralanması bir Contusive Modeli

While the majority of human spinal cord injuries occur in the cervical spinal cord, the vast majority of laboratory research employs animal models of ...

Controlled Cervical Laceration Injury in Mice

Farelerde Kontrollü Servikal yırtığı Sakatlık

Use of genetically modified mice enhances our  understanding of molecular mechanisms underlying several neurological disorders  such as a spinal cord ...

A Contusion Model of Severe Spinal Cord Injury in Rats

Sıçanlarda Şiddetli Spinal Kord Yaralanması bir bere modeli

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment (RMCA) in Individuals with Chronic Spinal Cord Injury

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment RMCA in Individuals with Chronic Spinal Cord Injury

Kronik Spinal Kord Yaralanması Bireylerde Solunum Motor Kontrol Değerlendirmesi (RMCA) kullanarak Solunum Kas Aktivasyon Değerlendirilmesi

During breathing, activation of respiratory muscles is coordinated by integrated input from the brain, brainstem, and spinal cord. When this coordination ...

Controlled Cortical Impact Model for Traumatic Brain Injury

Travmatik Beyin Hasarı için Kontrollü Kortikal Etki Modeli

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

A Radyo-telemetrik Sistemi Omurilik transeksiyonu ve Embriyonik Nöral Hücre Greftler Kök Sıçanlarda Kardiyovasküler Fonksiyonu Monitör

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

Deneysel Servikal Spinal Kord Yaralanmalarında Sinir Öncü Hücreler ve Öz-montaj Peptitler sinerjik Kullanımı

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

Calibrated Forceps Model of Spinal Cord Compression Injury

Omurilik Sıkıştırma Yaralanma Kalibre Forseps Modeli

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...