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DOI :
10.3791/51254-v
June 3rd, 2014
Chapters
0:05
Title
1:21
Cryo-electron Microscopy of Factor VIII Bound to Lipid Nanotubes
7:19
3D Reconstruction of Porcine Factor VIII Bound to Lipid Nanotubes
8:21
Electron Tomography of Porcine Factor VIII Bound to Lipid Nanotubes
9:42
Results: Factor VIII Helical Organization on Lipid Nanotubes
11:42
Conclusion
ヒトおよびブタ:我々は、2つの高度に相同なFVIII形態の膜結合構造を解決するために適用クライオ電子顕微鏡法、脂質ナノテクノロジー、および構造解析の組み合わせを提示する。螺旋状に負に荷電した脂質ナノチューブ(LNT)上の二つの機能の組換えFVIIIフォームを整理するために私たちの研究室で開発された方法論が記載されている。
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