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DOI :
10.3791/51255-v
•
9:13 min
April 30th, 2014
Chapters
0:05
Title
1:45
Spinal Cord and Brain Tissue Preparation
3:28
Myelin Flotation
4:33
Sucrose Gradient Fractionation
6:23
RNA Isolation from Individual Fractions
7:36
RNA Profiles from Spinal Cord and Brain Tissue
8:25
Conclusion
这个协议说明为了研究体内中枢神经系统的样本translatome多核糖分馏的必要修改。它允许翻译和转录调控的全球评估通过隔离和核糖体结合的RNA成分的总RNA的比较。
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