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DOI :
10.3791/51255-v
•
9:13 min
April 30th, 2014
Chapters
0:05
Title
1:45
Spinal Cord and Brain Tissue Preparation
3:28
Myelin Flotation
4:33
Sucrose Gradient Fractionation
6:23
RNA Isolation from Individual Fractions
7:36
RNA Profiles from Spinal Cord and Brain Tissue
8:25
Conclusion
このプロトコルは、in vivoでのCNSサンプルのtranslatomeを研究するためにポリリボソーム分画の本質的な変更を示している。これは、結合したRNA画分をリボソームへの全RNAの単離と比較を通じて翻訳と転写制御の全体的な評価が可能になります。
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