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DOI :
10.3791/51276-v
May 4th, 2014
Chapters
0:05
Title
2:05
Coverslip Preparation
2:48
Dissection of the Hippocampi
3:58
Cell Dissociation and Plating
5:35
Rat Hippocampal Primary Neuron Transfection using Lipofectamine
7:23
Immunostaining Hippocampal Primary Neurons
8:54
Dendritic Spine Imaging using Structure Illumination Microscopy
12:12
Results: Dendrites and Dendritic Spines Imaged with SIM and Confocal Microscopy
13:22
Conclusion
この記事では、構造化照明顕微鏡(SIM)を使用してインビトロで海馬ニューロンからイメージ樹状突起棘にワーキングプロトコルについて説明します。 SIMを使用して超解像顕微鏡法は、改善されたディテール個体樹状突起棘の画像化を可能にする、有意にすべての3つの空間次元における光の回折限界を超えて画像解像度を提供する。
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