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DOI :
10.3791/51278-v
•
16:26 min
June 28th, 2014
Chapters
0:05
Title
2:55
Isolation of Rat Brain Microvessels
9:27
Purification and Proliferation of RBEC Primocultures
10:32
Differentiation: Setting up the In Vitro BBB Model
14:50
Results: Immunocytochemical Characterization of the Rat In Vitro BBB Model and the Paracellular Permeability of LY across the Endothelial Monolayer
15:44
Conclusion
本研究的目的是要验证的体外血脑屏障模型涉及的大鼠同源内皮细胞和星形胶质细胞的共培养物的再现性。内皮细胞单层呈现高跨膜电阻和低LY渗透性。具体TJ蛋白的表达,功能反应,炎症和运输者和受体的功能进行了评估。
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