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DOI :
10.3791/51278-v
•
16:26 min
June 28th, 2014
Chapters
0:05
Title
2:55
Isolation of Rat Brain Microvessels
9:27
Purification and Proliferation of RBEC Primocultures
10:32
Differentiation: Setting up the In Vitro BBB Model
14:50
Results: Immunocytochemical Characterization of the Rat In Vitro BBB Model and the Paracellular Permeability of LY across the Endothelial Monolayer
15:44
Conclusion
本研究の目的は、ラット同系内皮細胞とアストロサイトの共培養が関与する生体外 BBBモデルの再現性を検証することでした。内皮細胞単層は、高いTEERと低LY透過性を提示した。特定のTJタンパク質の発現は、炎症および輸送体および受容体の機能性に機能的応答を評価した。
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