Name: a technique to functionalize and self-assemble macroscopic nanoparticle-ligand monolayer films onto template-free substrates
Uploaded: 2014-05-09T19:00:00
Description: Watch this Scientific Journal Video about A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates at JoVE.com

这项工作由海军研究办公室提供的资金得到了支持。 J.丰塔纳承认国家研究理事会博士后院士。

1，自组装配体纳米粒子单层膜作为自组装技术的，宏观的，巯基链烷封端的金纳米球单层膜是如下制作的说明性示例： <ol><li>集中15nm的金纳米球（可商购于许多密度：10 12个粒子/ ml）的水，以10〜13个粒子/ ml。 <ol><li>放置15毫升稀纳米球的水悬浮液中的成的超离心过滤器（100 K标称分子量限度）。 </li><li>离心过滤器/小瓶在4500×g离心2分钟，或直至只有几毫?...

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本协议描述了一个单独的“一锅煮”自组装技术来创建使用相转移，相分离和表面张力梯度宏观纳米粒子为配体的单层膜。这种技术的优点在于，它结合了三种自组装工艺成一个单一的，低成本的过程;通过快速有效地相转移纳米颗粒组装的颗粒进入细胞单层在气 - 液界面和运输单层薄膜上无模板基板。 用于创建高密度的单分子膜的最关键要素是使用新合成的柠檬酸盐稳定的?...

宏观的纳米颗粒薄膜的自组装已引起高度重视其从元件5的几何结构和组成决定的独特的性质，并且可能导致大范围的光学，电子和化学应用6-14。自组装等电影上铺配体的金属纳米颗粒必须被包装成高密度，单层。然而几个装配问题需要解决，以推动这类材料的发展。 首先，表面活性剂稳定化的金属纳米颗粒通常是通过在稀悬浮液15的湿化学方法合成。为了防止聚集，并控制在该薄膜中的纳米粒子的粒子间距，纳米粒子需要与配体壳为上限。经过纳米粒子进行了功能化与配体的纳米粒子通常保持在较稀的悬浮液。的技术是那么NEEDED自组装的纳米颗粒成宏观的，高密度，单层膜16，17。 使用巯基化的聚苯乙烯在水-四氢呋喃悬浮Cheng 等人 18相转移金纳米棒。其中，然后重新悬浮于氯仿和一滴放置在空气 - 水界面和纳米棒缓慢蒸发，形成的单层膜。 Bigioni 等 17创造了使用过量的配体和溶剂迅速挥发十二硫醇皑皑的黄金纳米微球的宏观单层，但纳米球需要是相前自组装转移。 一旦单层膜形成，他们通常需要被运送到基底上。 Mayya 等 3只限于纳米微球在水-甲苯接口，并使用表面张力梯度转移他们到无模板基板。同样，约翰逊<e采用表面张力梯度从两种不混溶流体米&gt;等人在过量配体4悬浮的银纳米球，然后翻译的纳米颗粒组成的小瓶的壁上。而组装技术存在解决所有这些问题，需要更有效的技术是必要的，大规模的纳米颗粒薄膜制作的发展提供帮助。 这里我们展示了一个简单的和强大的技术，它结合到一个单一的“一锅煮”的技术，在图1所示的上述三种自组装的问题。一种水混溶的有机溶剂（ 如四氢呋喃，dimeythl亚砜），用于第一快速有效地官能硫醇配位体（ 如巯基烷烃，硫醇-烯，巯基苯酚）到纳米颗粒（ 例如金的纳米球，纳米棒等）。该混合物，然后驱动所述纳米颗粒的自组装成宏观的，高密度，monola揭掉膜在空气 - 液体界面使用相分离。最后，纳米颗粒的单层膜形成到利用表面张力梯度从水/有机溶剂混合物中， 图2和图3无模板基板。

<table border="0" cellpadding="0" cellspacing="0" width="628">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">1-6 hexanedithiol</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">H12005-5G</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">1-dodecanethiol</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">471364-100ML</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">20 ml liquid scintillation vials</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">Z253081-1PAK</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:25px;width:224px;">acetone</td>
			<td style="width:97px;">Sigma</td>
			<td>650501-1L</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;width:224px;">amicon ultra-15 centrifugal filter&#160;</td>
			<td style="width:97px;">Millipore</td>
			<td style="width:140px;">100K</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">centrifuge</td>
			<td style="width:97px;">Sorvall&#160;</td>
			<td style="width:140px;">RC5B</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">centrifuge&#160;</td>
			<td style="width:97px;">Eppendorf</td>
			<td style="width:140px;">5810R</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">deionized water&#160;</td>
			<td style="width:97px;">in-house&#39;</td>
			<td style="width:140px;">N/A</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:25px;width:224px;">glass slides</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">CLS294875X25-72EA</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;width:224px;">15 nm gold nanospheres</td>
			<td style="width:97px;">Ted Pella, Inc</td>
			<td style="width:140px;">15703-1</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">hexamethyldisilazane</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">52619-50ML</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">hydrogen peroxide (30%)</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">216763-100ML</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">scanning electron microscope</td>
			<td style="width:97px;">Carl Zeiss</td>
			<td style="width:140px;">Model 55</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">polished silicon wafer</td>
			<td style="width:97px;">Sun Edison</td>
			<td style="width:140px;">N/A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">spectrometer</td>
			<td style="width:97px;">OceanOptics</td>
			<td style="width:140px;">USB4000-VIS-NIR</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">sulfuric acid</td>
			<td style="width:97px;">Fisher</td>
			<td style="width:140px;">A300-212</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">tetrahydrofuran</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">401757-100ML</td>
			<td></td>
		</tr>
	</tbody>
</table>

a technique to functionalize and self-assemble macroscopic nanoparticle-ligand monolayer films onto template-free substrates

这个协议描述了一种自组装技术来建立配体包覆的纳米微粒1,2组成的宏观单层膜。的简单，坚固且可扩展的技术有效地functionalizes金属纳米粒子与巯基配体在一个可混溶的水/有机溶剂混合物中，允许硫醇基团的快速接枝到纳米金表面。从水基悬浮液的纳米颗粒的疏水性配体然后迅速相分离的纳米颗粒，并将其限制到空气 - 液体界面。此驱动器的配位体覆盖的纳米颗粒，形成单层结构域在空气 - 液体界面。使用与水混溶的有机溶剂是重要的，因为它使来自接口的纳米颗粒的运输到无模板基板。该流量由表面张力梯度3,4介导的，并建立宏观的，高密度，单层nanop文章配体膜。此自组装技术可概括为包括使用不同的组成，大小的颗粒，和形状，并可能导致高效率的装配方法，以生产出低成本的，宏观的，高密度的，单层的纳米颗粒薄膜的广泛的应用。

图1（a）所示的金纳米球，巯基链烷配体，四氢呋喃和水的玻璃小瓶中混合后立即悬浮液。的概略的三个主要的自组装阶段，相转移，相分离，和表面张力梯度介导的膜输送示于图1（b）为在靠近所述小瓶的侧面的空气-液体界面的放大图。 上的配位体的硫醇基团的混合，置换的离子性表面活性剂，使微球成为疏水性的，更可混溶的THF后迅速结合到金?...

Watch this Scientific Journal Video about A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates at JoVE.com

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

一个简单，强大和可扩展的技术和功能化自组装纳米宏观 - 配体单层膜模板上的无基材在本协议中所述。

一种技术，功能化和自组装纳米宏观 - 配体单层膜模板上无基材

This protocol describes a self-assembly technique to create macroscopic monolayer films composed of ligand-coated nanoparticles1,2. The simple, robust and ...

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