Name: a technique to functionalize and self-assemble macroscopic nanoparticle-ligand monolayer films onto template-free substrates
Uploaded: 2014-05-09T19:00:00
Description: Watch this Scientific Journal Video about A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates at JoVE.com

Dette arbejde blev støttet med støtte leveres fra Office of Naval Research. J. Fontana anerkender National Research Council for en postdoc Associateship.

1.. Selvsamlede ligand-nanopartikel Encellelag Som et illustrativt eksempel på saml-selv teknik, makroskopiske, thiol-alkan udjævnede guld nanosphere monolagfilm produceres som følger: <ol><li> Koncentrer 15 nm guld nanospheres (kommercielt tilgængelige på en række tæthed: 10 12 partikler / ml) til ~ 10 13 partikler / ml i vand. <ol><li> Placer 15 ml af den fortyndede nanosphere vand suspensionen i en ultra centrifugal filter (100 K nominel molekylvægt grænse...

<ol>
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Denne protokol beskriver en enkelt &quot;one-pot&quot; saml-selv-teknik til at skabe makroskopiske nanopartikel-ligand monolagfilm hjælp fase overførsel, faseadskillelse og overfladespænding stigninger. Fordelen ved denne teknik er, at den kombinerer tre selvsamlingsproceser i en enkelt, billig proces; ved hurtigt og effektivt afvikle overføre nanopartikler, montage partiklerne i monolag ved luft-væske-grænsefladen og transport af monolagfilm onto skabelon-fri substrater. De mest kriti...

Den selv-samling af makroskopiske nanopartikel film har tiltrukket stor opmærksomhed for deres unikke egenskaber bestemmes ud fra geometri og sammensætning af elementerne 5 og kan føre til en lang række af optiske, elektroniske og kemiske applikationer 6-14. Selvstændige samle sådanne film metalliske nanopartikler udjævnet med ligander skal pakkes ind i high density, monolag. Men flere montage spørgsmål skal rettes til fremme udviklingen af ​​sådanne materialer. Først overfladeaktivt stabiliseret metalliske nanopartikler syntetiseres typisk ved våd-kemiske metoder i fortyndede suspensioner 15. For at forhindre aggregering og at kontrollere interparticle afstand af nanopartikler i filmene, skal udjævnet med ligand skaller nanopartikler. Når nanopartiklerne er funktionaliseret med ligander nanopartikler forbliver typisk i relativt fortyndede suspensioner. En teknik er så needed til selv samle nanopartikler i makroskopiske, high-density, monolagfilm 16, 17. Cheng et al. 18. fase overført guld nanorods hjælp thioleret polystyren i en vand-tetrahydrofuran-suspension. Nanorods hvor derefter igen suspenderet i chloroform og en dråbe blev placeret på en luft-vand-grænsefladen og inddampes langsomt, danner monolagfilm. Bigioni et al. 17. skabte makroskopiske monolog af dodecanthiol udjævnede guld nanospheres anvender overskydende ligand og hurtig fordampning af opløsningsmidlet, men nanokuglerne skulle være fase overført forud for selv-montage. Når monolagfilm dannes de typisk skal transporteres på et substrat. Mayya et al. 3 begrænset nanosfærer på et vand-toluen-interface og overførte dem på skabelon-fri underlag ved hjælp af overfladespænding gradienter. Tilsvarende Johnson <em&gt; et al. 4 ophængte sølv nanospheres i overskydende ligand og derefter oversat nanopartikler op væggene i hætteglasset ved hjælp af overfladespændingen gradienter fra to ikke-blandbare væsker. Mens samling teknikker eksisterer for at behandle hvert enkelt af disse spørgsmål er behov for mere effektive teknikker er nødvendig for at hjælpe med udviklingen af ​​storstilet nanopartikel filmproduktion. Her vi demonstrere en enkel og robust teknik, der kombinerer de tre selvsamling problemer beskrevet ovenfor i en enkelt &quot;one-pot&quot;-teknik, der er vist i fig. 1. En vand blandbart organisk opløsningsmiddel (fx tetrahydrofuran, dimeythl sulfoxid), anvendes til at først hurtigt og effektivt funktionalisere thiol-ligander (f.eks thiol-alkan-, thiol-en-, thiol-phenol) på nanopartikler (fx guld nanospheres, nanorods osv.). Blandingen driver så selvsamling af nanopartikler i makroskopiske, høj massefylde, Monolayer film på luft-væske grænsefladen ved hjælp af fase adskillelse. Endelig monolagfilm af nanopartikler dannes på skabelon-fri substrater ved hjælp af overfladespænding gradienter fra vand / organisk opløsningsmiddelblanding, figur 2 og figur 3.

<table border="0" cellpadding="0" cellspacing="0" width="628">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">1-6 hexanedithiol</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">H12005-5G</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">1-dodecanethiol</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">471364-100ML</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">20 ml liquid scintillation vials</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">Z253081-1PAK</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:25px;width:224px;">acetone</td>
			<td style="width:97px;">Sigma</td>
			<td>650501-1L</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;width:224px;">amicon ultra-15 centrifugal filter&#160;</td>
			<td style="width:97px;">Millipore</td>
			<td style="width:140px;">100K</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">centrifuge</td>
			<td style="width:97px;">Sorvall&#160;</td>
			<td style="width:140px;">RC5B</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">centrifuge&#160;</td>
			<td style="width:97px;">Eppendorf</td>
			<td style="width:140px;">5810R</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">deionized water&#160;</td>
			<td style="width:97px;">in-house&#39;</td>
			<td style="width:140px;">N/A</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:25px;width:224px;">glass slides</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">CLS294875X25-72EA</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;width:224px;">15 nm gold nanospheres</td>
			<td style="width:97px;">Ted Pella, Inc</td>
			<td style="width:140px;">15703-1</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">hexamethyldisilazane</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">52619-50ML</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">hydrogen peroxide (30%)</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">216763-100ML</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">scanning electron microscope</td>
			<td style="width:97px;">Carl Zeiss</td>
			<td style="width:140px;">Model 55</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">polished silicon wafer</td>
			<td style="width:97px;">Sun Edison</td>
			<td style="width:140px;">N/A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">spectrometer</td>
			<td style="width:97px;">OceanOptics</td>
			<td style="width:140px;">USB4000-VIS-NIR</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">sulfuric acid</td>
			<td style="width:97px;">Fisher</td>
			<td style="width:140px;">A300-212</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:224px;">tetrahydrofuran</td>
			<td style="width:97px;">Sigma</td>
			<td style="width:140px;">401757-100ML</td>
			<td></td>
		</tr>
	</tbody>
</table>

a technique to functionalize and self-assemble macroscopic nanoparticle-ligand monolayer films onto template-free substrates

Denne protokol beskriver en saml-selv teknik til at skabe makroskopiske monolagfilm sammensat af ligand-belagt nanopartikler 1, 2. Den enkle, robuste og skalerbare teknik effektivt functionalizes metalliske nanopartikler med thiol-ligander i en blandbar blanding af vand / organisk opløsningsmiddel, der giver mulighed for hurtig podning af thiolgrupper på guld nanopartikel overflade. De hydrofobe ligander på nanopartiklerne derefter hurtigt fase fraskilles nanopartikler fra vandbaserede suspension og begrænse dem til luft-væske-grænsefladen. Dette driver ligand-udjævnede nanopartikler til at danne monolag domæner ved luft-væske-grænsefladen. Brugen af ​​vandblandbare organiske opløsningsmidler er vigtigt, da det giver mulighed for transport af nanopartikler fra grænsefladen på skabelon-fri substrater. Strømningen er medieret af en overfladespænding gradient 3, 4 og skaber makroskopisk høj massefylde, monolag nanoparticle-ligand film. Denne Selvsamlingsproceduren teknik kan generaliseres til at omfatte brugen af ​​partikler af forskellige sammensætninger, størrelse og form og kan føre til en effektiv samling metode til at producere billige, makroskopiske, high-density, monolag nanopartikel film til udbredte applikationer .

Figur 1 (a) viser en suspension af guld nanospheres, thiol-alkan ligander, tetrahydrofuran og vand i et hætteglas umiddelbart efter blanding. En skematisk af de tre hovedfaser selvsamling, faseoverførings fase separation, og overfladespænding gradient medieret filmtransport er vist i figur 1 (b) som en udvidet visning på luft-væske grænsefladen nær siden af hætteglasset. De thiolgrupper på ligander hurtigt binde til guld nanospheres efter blanding f...

Watch this Scientific Journal Video about A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates at JoVE.com

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

En enkel, robust og skalerbar teknik til functionalize og selv samle makroskopiske nanopartikel-ligand monolagfilm på skabelon-fri underlag er beskrevet i denne protokol.

En teknik til at functionalize og selv samle Makroskopiske Nanopartikel-ligand monolagfilm på Skabelon-fri substrater

This protocol describes a self-assembly technique to create macroscopic monolayer films composed of ligand-coated nanoparticles1,2. The simple, robust and ...

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