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DOI :
10.3791/51284-v
May 22nd, 2014
Chapters
0:05
Title
1:34
Priming and Activating the Inflammasome
2:46
Sample Collection
5:05
Data Acquisition
6:52
Results: Measuring TLR8 Priming with R848
8:33
Conclusion
树突状细胞(DC)分泌IL-1β响应于TLR8识别合成嘌呤,R848,接着NLRP3炎性体激活与尼日利亚菌素,因此,IL-1β可以用来测量NLRP3炎性活性。细胞内细胞因子染色法,免疫印迹和ELISA法用于准确地测量通过IL-1β表达NLRP3炎性引发和活化。
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