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Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

DOI :

10.3791/51289-v

May 23rd, 2014

May 23rd, 2014

10,382 Views

1Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute, 2Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School

Quantitative measurement of bone progenitor function in fracture healing requires high resolution serial imaging technology. Here, protocols are provided for using intravital microscopy and osteo-lineage tracking to sequentially image and quantify the migration, proliferation and differentiation of endogenous osteogenic stem/progenitor cells in the process of repairing bone fracture.

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In Vivo Imaging

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