Name: human cartilage tissue fabrication using three-dimensional inkjet printing technology
Uploaded: 2014-06-10T13:45:00
Description: Watch this Scientific Journal Video about Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology at JoVE.com

作者要感谢来自纽约首都地区研究联盟资助的支持。

1，生物印刷平台建立打印机的修改是基于对HP DESKJET 500热喷墨​​打印机和HP 51626A黑色墨盒。 <ol><li>取下打印机顶部塑料盖，小心地从盖取下控制面板。 </li><li>拆下打印机的顶部和底部之间的3线连接。从底座上拆下打印机的顶部。 </li><li>在打印机顶部，取下墨盒下在右边的小塑料及橡胶配件（打印头清洗系统）。 </li><li>取出进纸托盘与弹簧的基础。 </li><li>取下金属板覆?...

<ol>
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这种3D生物印刷系统和同步光聚合产能提供比使用注射器挤出蜂窝海藻酸钠水凝胶16骨软骨缺损的原位印刷的最佳先前报道的方法一个显著更高的打印分辨率。较高的打印分辨率是特别关键的软骨组织工程恢复解剖软骨带状组织。层 - 层组装过程中同时进行光聚合反应是至关重要的，以维持细胞和生物材料支架的三维结构的精确沉积。微细加工与各印刷层也导致带状层之间的?...

生物印刷基于热喷墨印刷是最有前途的应用技术在组织工程和再生医学的领域之一。数字控制和高吞吐量的打印头单元，支架和生长因子可以精确地沉积到所需的二维（2D）和三维（3D）位置的迅速发展。采用该技术在组织工程和再生医学1-9许多成功的应用已经实现。在本文中，生物印刷一个平台，建立与修改后的惠普（HP）DESKJET 500热喷墨​​打印机和同步光聚合体系。由聚（乙二醇）（PEG）配制合成的水凝胶已经表明维持软骨细胞的生存力和促进软骨ECM生成10,11的容量。此外，光交联聚乙二醇在水中具有低粘度，这使得它非常适合同时聚合物：水溶性高在生物印刷3D化。在本文中，人类软骨细胞悬浮在聚（乙烯）乙二醇二丙烯酸酯（PEGDA; 3,400兆瓦）的精确打印，构建neocartilage层 - 层与1,400 dpi的三维解析。观察到沉积的细胞在三维支架材料中均匀分布，即生成软骨组织具有优异的机械性能和增强的ECM生成。与此相反，在手动制造的凝胶，而不是其最初沉积仓的底部由于较慢的聚合骨架，而导致不均匀的软骨形成培养后2,3蓄积的细胞。

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">HP Deskjet 500 thermal inkjet printer</td>
			<td style="width:221px;">Hewlett-Packard</td>
			<td style="width:195px;">C2106a</td>
			<td style="width:433px;">Discontinued. Purchased refurbished from internet vendor.</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">HP black ink cartridge</td>
			<td style="width:221px;">Hewlett-Packard</td>
			<td style="width:195px;">51626a</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Ultraviolet lamp</td>
			<td style="width:221px;">UVP</td>
			<td style="width:195px;">B-100AP</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">UV light meter</td>
			<td style="width:221px;">General Tools</td>
			<td style="width:195px;">UV513AB</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Zeiss LSM 510 laser scanning microscope</td>
			<td style="width:221px;">Carl Zeiss</td>
			<td style="width:195px;">LSM 510</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Dulbeccos Modified Eagles Medium (DMEM)</td>
			<td style="width:221px;">Mediatech</td>
			<td style="width:195px;">10-013</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Penicillin-streptomycin-glutamine (PSG)</td>
			<td style="width:221px;">Invitrogen</td>
			<td style="width:195px;">10378-016</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Accutase cell dissociation reagent</td>
			<td style="width:221px;">Invitrogen</td>
			<td style="width:195px;">A11105-01</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Phosphate buffered saline (PBS)</td>
			<td style="width:221px;">Invitrogen</td>
			<td style="width:195px;">10010-023</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Live/Dead viability/cytotoxicity Kit</td>
			<td style="width:221px;">Invitrogen</td>
			<td style="width:195px;">L-3224</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Poly(ethylene glycol) diacrylate (PEGDA)</td>
			<td style="width:221px;">Glycosan Biosystems</td>
			<td style="width:195px;">GS700</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Irgacure 2959</td>
			<td style="width:221px;">Ciba Specialty Chemicals</td>
			<td style="width:195px;">I-2959</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Human articular chondrocytes</td>
			<td style="width:221px;">Lonza</td>
			<td style="width:195px;">CC-2550</td>
			<td style="width:433px;"></td>
		</tr>
	</tbody>
</table>

human cartilage tissue fabrication using three-dimensional inkjet printing technology

生物印刷，这是基于热喷墨印刷，是最有吸引力的应用技术在组织工程和再生医学的领域之一。与数字控制单元，支架和生长因子可以精确地沉积到所需的二维（2D）和三维（3D）位置迅速。因此，这种技术是制造组织模仿他们的母语解剖结构的理想方法。为了与原生地带性的组织，细胞外基质成分（ECM）和机械性能工程师软骨，我们开发了使用商用喷墨打印机能够进行3D软骨组织工程同时进行光聚合生物印刷一个平台。人软骨细胞悬浮于聚（乙二醇）二丙烯酸酯（PEGDA），被印刷于通过层 - 层组装三维neocartilage施工。印刷细胞固定在原来的位置存放，由surro支持unding脚手架在同步光聚合。印刷组织的机械性能类似于天然软骨。相对于传统的组织的制造，这需要较长的紫外线曝光，在印刷单元和同步光聚合的存活率显著更高。印刷neocartilage展现了出色的糖胺聚糖（GAG）和Ⅱ型胶原的生产，这与基因的表达相一致。因此，这个平台是理想的精确细胞分布和安排解剖组织工程。

修改后的热喷墨打印机是能够用于细胞和支架材料沉积在高吞吐量和出色的细胞活力。与同时进行光致聚合和光敏生物材料相结合，这种技术能够以固定细胞和其他印刷物质的最初沉积的位置。根据修改后的热喷墨打印机的属性，在2D打印分辨率为300 dpi，采用130 PL单个墨滴体积。有50个发射喷嘴每个打印头与3.6 kHz的发射频率12,13。因此，对于直径4毫米和4毫米的高度，体积和各印刷层的层 ...

Watch this Scientific Journal Video about Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology at JoVE.com

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology

人体软骨组织制作采用三维喷墨打印技术

本文介绍的方法显示了如何将一个商用喷墨打印机与紫外线同时聚合生物印刷。该打印机能够构建三维组织结构，细胞和生物材料。这里展示的研究构建了一个3D neocartilage。

Bioprinting, which is based on thermal inkjet printing, is one of the most attractive enabling technologies in the field of tissue engineering and ...

Research

JoVE Journal

Bioengineering

Planar and Three-Dimensional Printing of Conductive Inks

平面和立体印刷的导电油墨

Printed electronics rely on low-cost, large-area fabrication routes to create flexible or multidimensional electronic, optoelectronic, and biomedical ...

科研

生物工程

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

三维全层皮肤的生成等效和自动伤人

In vitro models are a cost effective and ethical alternative to study cutaneous wound healing processes. Moreover, by using human cells, these models ...

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels

三维仿生技术：新型Biorubber创建自定义微观和宏观尺度架构的胶原凝胶

Tissue scaffolds play a crucial role in the tissue regeneration process. The ideal scaffold must fulfill several requirements such as having proper ...

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

倒胶体晶体聚（乙二醇）脚手架制作：肝脏组织工程三维细胞培养平台

The ability to maintain hepatocyte function in vitro, for the purpose of testing xenobiotics&#39; cytotoxicity, studying virus infection and developing ...

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

三维纸基微流体装置的制造用于免疫分析

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and ...

Melt Electrospinning Writing of Three-dimensional Poly(&#949;-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Melt Electrospinning Writing of Three-dimensional PolyÃƒÆ’Ã…Â½Ãƒâ€šÃ‚Âµ-caprolactone Scaffolds with Controllable Morphologies for Tissue Engineering Applications

用于组织工程应用的三维聚 (ε-内) 支架的熔体静电纺丝

This tutorial reflects on the fundamental principles and guidelines for electrospinning writing with polymer melts, an additive manufacturing technology ...

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

三维肽支架中哺乳动物细胞的培养

A useful technique for culturing cells in a self-assembling nanofiber three-dimensional (3D) scaffold is described. This culture system recreates an ...

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

三维热塑性材料的印刷, 以创建自动注射器泵的反馈控制微流控应用

Microfluidics has become a critical tool in research across the biological, chemical, and physical sciences. One important component of microfluidic ...

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

脱碳软骨衍生基质支架的制备

Osteochondral defects lack sufficient intrinsic repair capacity to regenerate functionally sound bone and cartilage tissue. To this extent, cartilage ...

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs

用于打印 3D 细胞-拉丹胰腺组织构造的胰腺组织衍生细胞外基质生物墨水

The transplantation of pancreatic islets is a promising treatment for patients who suffer from type 1 diabetes accompanied by hypoglycemia and secondary ...