Name: human cartilage tissue fabrication using three-dimensional inkjet printing technology
Uploaded: 2014-06-10T13:45:00
Description: Watch this Scientific Journal Video about Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology at JoVE.com

Forfatterne vil gerne anerkende den støtte fra New York Region Hovedstaden Research Alliance Grant.

1.. Bioprinting Platform Establishment Printeren ændring var baseret på en HP Deskjet 500 termisk inkjet printer og HP 51626A sort blækpatron. <ol><li> Fjern det øverste plastlåg på printeren og forsigtigt tages betjeningspanelet fra dækslet. </li><li> Frigør de 3 kabelforbindelser mellem printeren øverste del og base. Fjern printerens topdel fra basen. </li><li> På printerens øverste del, fjern lille plastik og gummi tilbehør (printhoved rengøring system) på højre side unde...

<ol>
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Denne 3D bioprinting-telefonsystem med simultan fotopolymeriserings kapacitet giver et væsentligt større udskrivning opløsning end den bedste tidligere rapporterede metode til in situ trykning af osteochondrale defekter ved hjælp af sprøjte ekstruderet et cellulært alginat hydrogel 16. Højere udskrivningsopløsningen er særlig kritisk for brusk tissue engineering for at genskabe den anatomiske brusk zone organisation. Samtidig fotopolymerisation under lag af lag-samling er afgørende for at o...

Bioprinting baseret på termisk inkjet trykning er en af ​​de mest lovende teknologier, der muliggør inden for tissue engineering og regenerativ medicin. Med digital kontrol og high throughput printhoveder celler, stilladser og vækstfaktorer kan nøjagtigt deponeres til den ønskede todimensionale (2D) og tre-dimensionelle (3D) positioner hurtigt. Mange succesfulde ansøgninger er blevet opnået ved hjælp af denne teknologi i tissue engineering og regenerativ medicin 1-9. I dette papir, blev en bioprinting platform etableret med en modificeret Hewlett-Packard (HP) Deskjet 500 termisk inkjet printer og en samtidig fotopolymeriserings system. Syntetiske hydrogeler er formuleret ud fra poly (ethylenglycol) (PEG) har vist evne til at opretholde chondrocyt levedygtighed og fremme chondrogenese ECM produktion 10,11. Desuden fotokrydsbindbart PEG er letopløseligt i vand med lav viskositet, hvilket gør det ideelt til samtidig polymefinitioner, godkendelse under 3D-bioprinting. I dette papir, menneskelige chondrocytter suspenderet i poly (ethylen) glycol diacrylat (PEGDA, MW 3.400), blev netop trykt at konstruere neocartilage lag-for-lag med 1.400 dpi i 3D opløsning. Homogen fordeling af deponerede celler i en 3D stillads blev observeret, hvilket genererede bruskvæv med fremragende mekaniske egenskaber og forbedret ECM produktion. Derimod i manuel fremstilling celler akkumulerede i bunden af gelen i stedet for deres oprindeligt deponeret positioner grund langsommere stillads polymerisation, hvilket førte til inhomogen bruskdannelse efter dyrkning 2,3.

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">HP Deskjet 500 thermal inkjet printer</td>
			<td style="width:221px;">Hewlett-Packard</td>
			<td style="width:195px;">C2106a</td>
			<td style="width:433px;">Discontinued. Purchased refurbished from internet vendor.</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">HP black ink cartridge</td>
			<td style="width:221px;">Hewlett-Packard</td>
			<td style="width:195px;">51626a</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Ultraviolet lamp</td>
			<td style="width:221px;">UVP</td>
			<td style="width:195px;">B-100AP</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">UV light meter</td>
			<td style="width:221px;">General Tools</td>
			<td style="width:195px;">UV513AB</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Zeiss LSM 510 laser scanning microscope</td>
			<td style="width:221px;">Carl Zeiss</td>
			<td style="width:195px;">LSM 510</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Dulbeccos Modified Eagles Medium (DMEM)</td>
			<td style="width:221px;">Mediatech</td>
			<td style="width:195px;">10-013</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Penicillin-streptomycin-glutamine (PSG)</td>
			<td style="width:221px;">Invitrogen</td>
			<td style="width:195px;">10378-016</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Accutase cell dissociation reagent</td>
			<td style="width:221px;">Invitrogen</td>
			<td style="width:195px;">A11105-01</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Phosphate buffered saline (PBS)</td>
			<td style="width:221px;">Invitrogen</td>
			<td style="width:195px;">10010-023</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Live/Dead viability/cytotoxicity Kit</td>
			<td style="width:221px;">Invitrogen</td>
			<td style="width:195px;">L-3224</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Poly(ethylene glycol) diacrylate (PEGDA)</td>
			<td style="width:221px;">Glycosan Biosystems</td>
			<td style="width:195px;">GS700</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Irgacure 2959</td>
			<td style="width:221px;">Ciba Specialty Chemicals</td>
			<td style="width:195px;">I-2959</td>
			<td style="width:433px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:357px;">Human articular chondrocytes</td>
			<td style="width:221px;">Lonza</td>
			<td style="width:195px;">CC-2550</td>
			<td style="width:433px;"></td>
		</tr>
	</tbody>
</table>

human cartilage tissue fabrication using three-dimensional inkjet printing technology

Bioprinting, som er baseret på termisk inkjet print, er en af ​​de mest attraktive støtteteknologier inden for tissue engineering og regenerativ medicin. Med digital kontrol celler, stilladser og vækstfaktorer kan nøjagtigt deponeres til den ønskede todimensionale (2D) og tre-dimensionelle (3D) placeringer hurtigt. Derfor er denne teknologi er en ideel metode til at fabrikere væv efterligner deres oprindelige anatomiske strukturer. For at konstruere brusk med indfødte zoner organisation, ekstracellulær matrix sammensætning (ECM) og mekaniske egenskaber, har vi udviklet et bioprinting platform ved hjælp af en kommerciel inkjet printer med samtidig fotopolymerisation stand til 3D-brusk tissue engineering. Humane chondrocytter suspenderet i poly (ethylenglycol) diacrylat (PEGDA) blev trykt for 3D neocartilage konstruktion via lag-for-lag forsamling. De trykte celler blev fastsat til deres oprindelige deponerede positioner, støttet af o.INANSIERING stillads i samtidige fotopolymerisation. De mekaniske egenskaber af det trykte væv svarede til det native brusk. Sammenlignet med konventionelle væv fabrikation, som kræver længere UV-eksponering, levedygtigheden af ​​de trykte celler med simultan fotopolymerisation var betydeligt højere. Trykt neocartilage demonstreret fremragende glycosaminoglycan (GAG) og kollagen type II produktion, hvilket var i overensstemmelse med genekspression. Derfor er denne platform er ideel for præcis celle distribution og arrangement for anatomisk tissue engineering.

Den modificerede termisk inkjet printer var i stand til celle og stillads deposition på high throughput og fremragende levedygtighed celle. Ved at kombinere med samtidig fotopolymerisation og lysfølsomme biomaterialer, denne teknologi er i stand til at løse de celler og andre trykte stoffer til de oprindeligt deponerede placeringer. Ifølge de egenskaber af den modificerede termisk inkjet printer, 2D udskrivningsopløsningen var 300 dpi med en enkelt blæk dråbe volumen på 130 pl. Der er 50 fyring dyser i hvert pri...

Watch this Scientific Journal Video about Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology at JoVE.com

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology

Menneskelige bruskvæv Fabrication Brug Tre-dimensionel Inkjet Printing Technology

De er beskrevet i dette papir metoder viser, hvordan man kan konvertere en kommerciel inkjet printer til en bioprinter med samtidig UV polymerisering. Printeren er i stand til at konstruere 3D-væv struktur med celler og biomaterialer. Undersøgelsen viste her opbygget en 3D neocartilage.

Bioprinting, which is based on thermal inkjet printing, is one of the most attractive enabling technologies in the field of tissue engineering and ...

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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Generation af en tre-dimensionel fuld tykkelse Skin Equivalent og Automated Anskydning

In vitro models are a cost effective and ethical alternative to study cutaneous wound healing processes. Moreover, by using human cells, these models ...

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels

Tre-dimensionel Biomimetic Teknologi: Novel Biorubber Opretter Defineret mikro- og makro-skala Architectures i Collagen Hydrogeler

Tissue scaffolds play a crucial role in the tissue regeneration process. The ideal scaffold must fulfill several requirements such as having proper ...

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Fabrikation af Inverted Kolloid Crystal Poly (ethylenglycol) Stillads: En Tre-dimensionel Cell Culture Platform for levervæv Engineering

The ability to maintain hepatocyte function in vitro, for the purpose of testing xenobiotics&#39; cytotoxicity, studying virus infection and developing ...

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fremstilling af Tre-dimensionel papir-baserede mikrofluidenheder for Immunoassays

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and ...

Melt Electrospinning Writing of Three-dimensional Poly(&#949;-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Melt Electrospinning Writing of Three-dimensional PolyÃƒÆ’Ã…Â½Ãƒâ€šÃ‚Âµ-caprolactone Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Smelte Electrospinning skrivning af tre-dimensionelle Poly(ε-caprolactone) stilladser med kontrollerbare morfologier for Tissue Engineering applikationer

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Dyrkning pattedyrsceller i tre-dimensionelle peptid stilladser

A useful technique for culturing cells in a self-assembling nanofiber three-dimensional (3D) scaffold is described. This culture system recreates an ...

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Tre-dimensionelle udskrivning af termoplastiske materialer til at oprette automatiseret sprøjte pumper med Feedback kontrol for mikrofluid programmer

Microfluidics has become a critical tool in research across the biological, chemical, and physical sciences. One important component of microfluidic ...

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Fabrikation af Decellularized brusk-afledte Matrix stilladser

Osteochondral defects lack sufficient intrinsic repair capacity to regenerate functionally sound bone and cartilage tissue. To this extent, cartilage ...

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The transplantation of pancreatic islets is a promising treatment for patients who suffer from type 1 diabetes accompanied by hypoglycemia and secondary ...