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DOI :
10.3791/51309-v
May 3rd, 2014
Chapters
0:05
Title
1:11
Collecting Nuclear Extract
4:09
Probe Preparation and PAGE Extraction
5:29
3' mRNA Cleavage Reaction and PAGE Analysis
8:04
Cleavage Assay of RNA poly(A) for HIV-1
8:44
Conclusion
RNAポリメラーゼIIは、成熟mRNAの3 '末端を越えて延びる前駆体RNAを合成する。成熟したRNAの末端が切断複合体のエンドヌクレアーゼ活性を経由して、RNA配列によって決定されるサイトで、cotranscriptionally生成されます。ここでは、in vitroでの切断反応を研究する方法を詳細に私たち。
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