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DOI :
10.3791/51320-v
April 3rd, 2014
Chapters
0:05
Title
1:25
Cell Culture on Collagen Coated Glass Disk
3:44
Cell Fixation
5:24
TM4SF1 Immunofluorescence Staining of Nanopodia
7:25
Results: Tracking the Nanopodia
10:07
Conclusion
Nanopodia很瘦但很脆弱的膜通道,向上延伸至100微米的细胞的领先的前面或后面尾随和检测的细胞环境。直接固定在较高的Triton X-100浓度为37℃,轻柔洗涤,并避免如乙醇,甲醇,丙酮或有机溶剂,并须遵守这些细胞结构。
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