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DOI :
10.3791/51330-v
•
11:38 min
March 21st, 2014
Chapters
0:05
Title
1:59
Cytokine Stimulation of Endothelial Cells
4:11
Isolation and Pretreatment of Peripheral Blood Lymphocytes
6:09
Setup of Flow Assay System
8:33
Flow AssayTechnique and Recording of Adhesion for Analysis
9:35
Results: Adhesion Cascade of Blood Lymphocytes over Hepatic Sinusoidal Endothelium
10:44
Conclusion
肝臓への白血球の動員は、固有の肝臓類洞内皮細胞で裏打ちされている肝正弦波の特殊なチャネル内に発生します。生理学的な剪断応力の条件下でヒト肝臓シヌソイド内皮を通過する白血球動員の位相差顕微鏡は、このプロセスの根底にある分子機構の解明を容易にすることができる。
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