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DOI :
10.3791/51334-v
•
12:28 min
April 2nd, 2014
Chapters
0:05
Title
1:57
pTIRF System Setup
5:54
Chromaffin Cell Isolation
7:04
pTIRF Image Acquisition
9:14
Results: Monitoring Membrane Deformations with pTIRFM
11:58
Conclusion
基于偏振的全内反射荧光显微镜(pTIRFM)实现实时检测细胞膜动力学。本文介绍pTIRFM的膜重塑的过程中调节胞吐作用的研究落实。该技术推广到在细胞生物学中的其他进程直接或间接地涉及改变膜的形状。
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