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DOI :
10.3791/51343-v
April 8th, 2014
Chapters
0:05
Title
1:43
Gentamicin-induced Hair Cell Necrosis and Subsequent Recovery Incubation
2:40
Vital Dye Staining of Fish for Fluorescent Analysis of Regenerating Neuromasts
3:30
Anesthetizing Fish and Counting Fluorescent Neuromasts
4:36
High Resolution Fluorescent Counting of Individual Hair Cells
6:01
Results: Lateral Line Regenerations Assay
9:02
Conclusion
由于神经和非神经系统疾病的许多斑马鱼模型研究在成年鱼,而不是胚胎/幼虫,我们开发了可应用于成年斑马鱼疾病模型的定量侧线再生试验。该法涉及的分辨率在1)神经丘和2)单个毛细胞的水平。
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