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DOI :
10.3791/51356-v
April 17th, 2014
Chapters
0:05
Title
1:49
Collection of E9.5 and E10.5 Embryos for Ex vivo Injection
3:10
Ex vivo DNA or Cell Injection Under a Stereomicroscope
4:35
Results: Embryonic Heart Cell Labeling and Injection
6:30
Conclusion
我们描述了一系列的方法来注入染料,DNA载体,病毒和细胞,以监测来自于胚胎或多能干细胞内的小鼠胚胎在胚胎天(E)9.5和后期阶段内源性和移植细胞的两个细胞命运和表型的发展。
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