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DOI :
10.3791/51387-v
April 23rd, 2014
Chapters
0:05
Title
1:14
Preparing Large Apple Juice Agar Plates
2:04
Collecting Embryos and Pre-crosslinking Treatment
4:35
In vivo Crosslinking
5:34
Preparing Proteins and Immunoprecipitation
7:10
Results: Crosslinked Tud Protein Complex Analysis
8:23
Conclusion
多成分タンパク質複合体は、細胞機能および開発中に重要な役割を果たしている。ここでは、後続の構造-機能分析のための架橋された複合体の精製に続いてインビボ架橋後のショウジョウバエ胚から天然のタンパク質複合体を単離するために用いた方法を記載している。
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