Name: cell-based therapy for heart failure in rat: double thoracotomy for myocardial infarction and epicardial implantation of cells and biomatrix
Uploaded: 2014-09-22T13:00:00
Description: Watch this Scientific Journal Video about Cell-based Therapy for Heart Failure in Rat: Double Thoracotomy for Myocardial Infarction and Epicardial Implantation of Cells and Biomatrix at JoVE.com

作者感谢教授亨德里克Tevaearai心血管外科为研究的初始部分资金支持的部门。

注：刘易斯雄性和雌性大鼠，200〜220克的安置标准实验室条件下（12小时光照和黑暗周期， 随意的水和食物，IVC笼）下。所有动物均符合FELASA和瑞士法律对动物保护的建议处理。 1，细胞的制备：从骨髓间充质干细胞分离<ol><li>麻醉大鼠用异氟烷5％和O 2的5升/分钟的感应腔室5分钟。将动物的口鼻部到鼻锥相连的麻醉系统。执行脚趾或尾巴捏确认镇静。 </li><li>?...

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法援署的永久结扎造成不可逆的心肌损伤。第一个动物模型于1960年4被描述。从那时起，它已被认为是慢性心肌梗塞标准和合适的模型。其稳定性和重复性使治疗的实验评价心肌梗死5。继最初的描述改进程序报告了一个手术死亡率35-13％6。 正如预期的那样，LAD结扎诱导的2个星期内观察到受损的心脏功能。在心脏功能的进一步下降，记录长达6周，并与...

自19 世纪结束时，心血管疾病一直留在工业化国家的头号连环杀手。其中，冠状动脉疾病代表了主要病因。急性期会导致心肌梗死（MI），随后是不适应的重塑是逐步走向慢性期和严重的心脏衰竭的发展。尽管最近显著技术和治疗的进步，发病率和死亡率因心脏衰竭的进展仍在增长1。在这种情况下，细胞疗法已经获得了越来越多的关注作为一种新的治疗方案，以阻止疾病朝向心脏衰竭的恶化和刺激心肌的最近确定的再生能力。实验和临床研究已经提供的各种细胞类型的心脏移植后获得的有益效果令人信服的证据。主要成果包括改进CARDI交流收缩功能降低，左心室重塑，减少梗塞面积，并在梗死区血管密度增加。然而，细胞注射后低细胞数量保持仍然是一个重要的缺点。细胞与生物基质，以改善电池交付2协会最近促成研究者和临床利益。 左冠状动脉前降支（LAD）结扎是心肌梗死的小动物模型，导致透梗死和成熟的疤痕的参考方法。在心肌梗死慢性期应用细胞治疗还需要第二个手术干预。正中胸骨切开术通常执行，使心肌内注射细胞或biografts的心外膜植入。这种微创外科手术增加的死亡率，手术后的恢复时间，疼痛和感染的风险。这里介绍的微创手术方法不仅可以防止这样的偏见，但也提供了Ô心脏的治疗应用ptimal访问。心肌梗死和凝胶型生物基质相关的细胞外膜植入是通过左肋间开胸手术在跳动的心脏进行。

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			<td height="21" style="height:21px;width:216px;">SURGIPRO II 7-0 Suture</td>
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			<td height="21" style="height:21px;width:216px;">CATHETER INSYTE 14G</td>
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			<td height="42" style="height:42px;width:216px;">INSPIRA ADVANCED VOLUME CONTROLLED VENTILATOR</td>
			<td style="width:104px;">HARVARD APPARATUS</td>
			<td style="width:119px;">557058</td>
			<td></td>
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			<td height="21" style="height:21px;width:216px;">DUMONT #7 Forceps</td>
			<td style="width:104px;">FST Germany</td>
			<td style="width:119px;">11274-20</td>
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			<td height="21" style="height:21px;width:216px;">Fibrin sealant</td>
			<td style="width:104px;">BAXTER</td>
			<td style="width:119px;">1501441</td>
			<td>TISSEEL Glue, Frozen -20&#176;C</td>
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			<td height="42" style="height:42px;width:216px;">CASTROVIEJO EYE SPECULA</td>
			<td style="width:104px;">HARVARD APPARATUS</td>
			<td style="width:119px;">72-8925</td>
			<td>use as retractor for the ribs</td>
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			<td height="21" style="height:21px;">IMDM Glutamax</td>
			<td>Gibco</td>
			<td>31980</td>
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			<td height="21" style="height:21px;">Pen/Strep</td>
			<td>Gibco</td>
			<td>15140</td>
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			<td height="21" style="height:21px;">FBS</td>
			<td>PAA Clone</td>
			<td>&#160;&#160;&#160;&#160;&#160; A15-101</td>
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			<td height="42" style="height:42px;">bone scissors</td>
			<td style="width:104px;">Fine Science Tols</td>
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			<td height="21" style="height:21px;">Red Blood Cell Lysis Solution</td>
			<td>Gentra Systems</td>
			<td>D-50k1</td>
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			<td height="42" style="height:42px;width:216px;">Accutase cell detachment solution</td>
			<td style="width:104px;">Stem cell technology</td>
			<td style="width:119px;">7920</td>
			<td></td>
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cell-based therapy for heart failure in rat: double thoracotomy for myocardial infarction and epicardial implantation of cells and biomatrix

心脏细胞疗法已经获得了越来越多的关注与细胞相关的生物材料和植入已成为一个主要的问题，优化心肌细胞传递。心肌梗死的啮齿动物模型（MI）由左前降支（LAD）结扎已普遍被通过开胸进行的;在传统上一直进行用于治疗心外膜应用程序通过胸骨第二心脏直视手术。由于LAD结扎模型的描述，手术后的死亡率已经从35-13％下降，但第二次手术依然是至关重要的。为了改善手术后的恢复，减少疼痛和感染，微创手术过程给出。两个开胸被执行，初始一个用于LAD结扎，而第二个用于治疗心外膜给药。 Biografts包括与固体或凝胶型的矩阵相关联的单元的方式进行涂敷在梗塞芳EA。 LAD结扎导致心脏功能的丧失证实了2周，6周后进行超声心动图。 Goldner对心脏切片进行三色染色证实透瘢痕形成。第一和第二次手术产生了小于10％，术后死亡率。

开胸手术后1小时内的所有动物恢复。伤口愈合快。无感染或水肿观察。 双左侧开胸允许的心脏最优访问（ 图1）。疼痛和手术后的死亡率较低。动物从手术中恢复迅速和体重增加（ 图2）。卡普兰梅厄成活率为96％，为第一开胸手术。四只超过104 通过第二开胸手术24小时以下处理应用程序内死亡。卡普兰-梅尔存活百分比从当动物接受第二次开?...

Watch this Scientific Journal Video about Cell-based Therapy for Heart Failure in Rat: Double Thoracotomy for Myocardial Infarction and Epicardial Implantation of Cells and Biomatrix at JoVE.com

Cell-based Therapy for Heart Failure in Rat: Double Thoracotomy for Myocardial Infarction and Epicardial Implantation of Cells and Biomatrix

基于细胞治疗心脏衰竭大鼠：双开胸心肌梗死和细胞与生物基质植入心外膜

àbiograft植入治疗心肌梗死的啮齿动物模型由法援署结扎诱发了常规需要两个开放式心脏手术。为了降低死亡率，并提供用于治疗与细胞相关的固体和胶状biomatrices固定最佳条件，微创手术已得到了发展。

Cardiac cell therapy has gained increasing interest and implantation of biomaterials associated with cells has become a major issue to optimize myocardial ...

Research

JoVE Journal

Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT

生物发光成像评估的免疫反应，植入心脏组织工程（东隧）

Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or ...

科研

生物工程

Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart

在小鼠心脏的动作电位和钙瞬变的光学测绘

The mouse heart is a popular model for cardiovascular studies due to the existence of low cost technology for genetic engineering in this species. ...

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

在肌肉再生的老鼠模型中移植诱导多能干细胞衍生的中生代细胞样肌原体

Patient-derived iPSCs could be an invaluable source of cells for future autologous cell therapy protocols. iPSC-derived myogenic stem/progenitor cells ...

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

管式膜网络在心肌细胞的心房和心室分析

In cardiac myocytes a complex network of membrane tubules - the transverse-axial tubule system (TATS) - controls deep intracellular signaling functions. ...

Biaxial Mechanical Characterizations of Atrioventricular Heart Valves

房室心脏瓣膜的双轴力学特性

Extensive biaxial mechanical testing of the atrioventricular heart valve leaflets can be utilized to derive&#160;optimal parameters used in constitutive ...

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

小鼠模型中生物合成组织工程气管移植的育苗与植入

Treatment options for congenital or secondary long segment tracheal defects have historically been limited due to an inability to replace functional ...

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

二维和三维 i 型胶原蛋白基质角质形成细胞增殖的评价

Type I collagen, useful as a substrate for cell culture, exists in two forms: the two-dimensional, non-fibrous form and three-dimensional, fibril form. ...

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy

磁性、声学和光学三响应性微泡，用于磁性高温和波托托热组合癌症治疗

The precision delivery of anti-cancer agents which aim for targeted and deep-penetrated delivery as well as a controlled release at the tumor site has ...

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

从人体 iPS 细胞中提取的网状工程心脏组织用于活体心肌修复

The current protocol describes methods to generate scalable, mesh-shaped engineered cardiac tissues (ECTs) composed of cardiovascular cells derived from ...

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

心肌梗塞穆林模型中的 3D 生物打印贴片移植

Testing regenerative properties of 3D bioprinted cardiac patches in vivo using murine models of heart failure via permanent left anterior descending (LAD) ...