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DOI :
10.3791/51401-v
•
12:59 min
May 1st, 2014
Chapters
0:05
Title
1:54
Sporulating Library of Transformed Cells to Obtain Barcoded Tetrads
3:29
Establishing the FACS Gates for Tetrad Sorting
6:14
Sorting Tetrads Onto an Agar Plate
8:16
Separating Spores
9:25
Harvesting Colonies
10:54
Results: BEST is an Efficient Way to Isolate Spores from Tetrads
12:37
Conclusion
四分体启用条码序列(BEST)取代分离,破坏和间距四分体的手动流程。的最优隔离四分体通过荧光激活细胞分选到琼脂平板上,通过搅拌与玻璃珠分离出孢子,并确定哪个随机排列菌落,从同一原始四分使用分子条形码而得。
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