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DOI :
10.3791/51415-v
July 29th, 2014
Chapters
0:05
Title
2:25
Infection of A549 Cells with Rift Valley Fever Virus Clone 13 (Cl 13)
4:17
Preparation od Cell Lysates
5:47
Determination of Conformational Changes of Pattern Recognition Receptors
8:40
Analysis of Oligomeric States of Pattern Recognition Receptors
9:46
Results: RIG-I and PKR Assay
12:00
Conclusion
先天防御病毒感染是由模式识别受体(模式识别受体)触发。这两个细胞质模式识别受体RIG-I和PKR结合到病毒签名的RNA,构象变化,低聚,并激活抗病毒信号。方法被描述,其允许方便地监控构象交换和这些细胞质的PRR的低聚。
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