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DOI :
10.3791/51420-v
•
14:53 min
May 13th, 2014
Chapters
0:05
Title
2:14
Cell Culture and Treatment
4:58
Click-iT Assay
9:59
Light Microscopy and Data Analysis
12:41
Results: RNA Expression in Hypoxic Cells
14:02
Conclusion
我们描述了全球性RNA合成缺氧使用成像分析技术。以前没有缺氧条件下进行的RNA点击化学标记,并允许全球的RNA变化的可视化在单细胞水平。这种方法将与现有的平均值RNA技术,允许的细胞 - 细胞间的变化在全球RNA合成的直接可视化。
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