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DOI :
10.3791/51425-v
June 17th, 2014
Chapters
0:05
Title
1:41
Depletion from Xenopus Extract
2:57
Optimizing Xenopus Extract for β-catenin Degradation
4:10
Radiolabeled β-catenin Degradation Assay in Xenopus Egg Extract
5:47
β-catenin-luciferase Degradation Assay in Xenopus Egg Extract
7:24
Results: Analysis of β-catenin Degradation
9:03
Conclusion
방법은 Xenopus의 계란 추출물 및 단백질 분해의 저분자 변조기에 대한 고 처리량 스크리닝을 위해 그 적응 방사성 표지와 루시퍼 라제 융합 단백질을 이용하여 단백질 분해 분석에 대해 설명한다.
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