Name: a full skin defect model to evaluate vascularization of biomaterials in vivo
Uploaded: 2014-08-28T14:00:00
Description: Watch this Scientific Journal Video about A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo at JoVE.com

Integra真皮再生模板，请通过Integra生命科学公司提供。资金配套工作来源：（上午十时正15090007），这项工作由CIRM，BMBF的早期平移二奖及FONDAP中心基因组调控既要JTE了部分资金。

1，准备的支架<ol><li>通过使用12毫米活检穿孔产生的支架的样品。 </li><li>引进生物活性分子或细胞进入支架，轻轻挤压它们，用无菌纱布引流支架。然后通过加入160微​​升含有生物活性分子或所关注的细胞的溶液中再水化的支架。仔细检查，通过代谢试验，如MTT法生物活化与细胞的成功。 </li><li>如果需要的话，固定的化合物或细胞可以在支架内测试了通过在纤维蛋白的​​凝血酶溶液或水凝...

<ol>
	<li>Rahaman, M. N., Mao, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stem+cell-based+composite+tissue+constructs+for+regenerative+medicine.">Stem cell-based composite tissue constructs for regenerative medicine.</a> Biotechnology and Bioengineering. 91 (3), 261-284 (2005).</li><li>Lutolf, M. P., Hubbell, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthetic+biomaterials+as+instructive+extracellular+microenvironments+for+morphogenesis+in+tissue+engineering.">Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering.</a> Nature Biotechnology. 23, 47-55 (2005).</li><li>Machens, H. G., Berger, A. C., Mailaender, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioartificial+skin.">Bioartificial skin.</a> Cells Tissues Organs. 167, 88-94 (2000).</li><li>Priya, S. G., Jungvid, H., Kumar, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Skin+tissue+engineering+for+tissue+repair+and+regeneration.">Skin tissue engineering for tissue repair and regeneration.</a> Tissue Engineering Part B: Reviews. 14, 105-118 (2008).</li><li>Papavasiliou, G., Cheng, M. H., Brey, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strategies+for+vascularization+of+polymer+scaffolds.">Strategies for vascularization of polymer scaffolds.</a> Journal of Investigative Medicine. 58 (7), 838-844 (2010).</li><li>Laschke, M. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Angiogenesis+in+tissue+engineering:+breathing+life+into+constructed+tissue+substitutes.">Angiogenesis in tissue engineering: breathing life into constructed tissue substitutes.</a> Tissue Engineering. 12, 2093-2104 (2006).</li><li>Zhong, S. P., Zhang, Y. Z., Lim, C. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue+scaffolds+for+skin+wound+healing+and+dermal+reconstruction.">Tissue scaffolds for skin wound healing and dermal reconstruction.</a> Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology. 2 (5), 510-525 (2010).</li><li>Liu, G., Zhang, Y., Liu, B., Sun, J., Li, W., Cui, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bone+regeneration+in+a+canine+cranial+model+using+allogeneic+adipose+derived+stem+cells+and+coral+scaffold.">Bone regeneration in a canine cranial model using allogeneic adipose derived stem cells and coral scaffold.</a> Biomaterials. 34 (11), 2655-2664 (2013).</li><li>Hansson, A., Di Francesco, T., Falson, F., Rousselle, P., Jordan, O., Borchard, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preparation+and+evaluation+of+nanoparticles+for+directed+tissue+engineering.">Preparation and evaluation of nanoparticles for directed tissue engineering.</a> International Journal of Pharmaceutics. 439 (1-2), 73-80 (2012).</li><li>Sarkar, S. D., Farrugia, B. L., Dargaville, T. R., Dhara, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chitosan-collagen+scaffolds+with+nano/microfibrous+architecture+for+skin+tissue+engineering.">Chitosan-collagen scaffolds with nano/microfibrous architecture for skin tissue engineering.</a> Journal of Biomedical Materials Research Part A. 18, (2013).</li><li>Wang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+roles+of+knitted+mesh-reinforced+collagen-chitosan+hybrid+scaffold+in+the+one-step+repair+of+full-thickness+skin+defects+in+rats.">The roles of knitted mesh-reinforced collagen-chitosan hybrid scaffold in the one-step repair of full-thickness skin defects in rats.</a> Acta Biomaterials. 9 (8), 7822-7832 (2013).</li><li>Rizzi, S. C., Upton, Z., Bott, K., Dargaville, T. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+advances+in+dermal+wound+healing:+biomedical+device+approaches.">Recent advances in dermal wound healing: biomedical device approaches.</a> Expert Review of Medical Devices. 1, 143-154 (2010).</li><li>des Rieux, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3D+systems+delivering+VEGF+to+promote+angiogenesis+for+tissue+engineering.">3D systems delivering VEGF to promote angiogenesis for tissue engineering.</a> Journal of Controlled Release. 150, 272-278 (2011).</li><li>Reckhenrich, A. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioactivation+of+dermal+scaffolds+with+a+non-viral+copolymer-protected+gene+vector.">Bioactivation of dermal scaffolds with a non-viral copolymer-protected gene vector.</a> Biomaterials. 32, 1996-2003 (2011).</li><li>Chen, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Key+Regulatory+Roles+of+the+PI3K/Akt+Signaling+Pathway+in+the+Functionalities+of+Mesenchymal+Stem+Cells+and+Applications+in+Tissue+Regeneration.">The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration.</a> Tissue Engineering Part B Rev. 19, 516-528 (2013).</li><li>Fedorovich, N. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+endothelial+progenitor+cells+in+prevascularized+bone+tissue+engineering:+development+of+heterogenous+constructs.">The role of endothelial progenitor cells in prevascularized bone tissue engineering: development of heterogenous constructs.</a> Tissue Engineering Part A. 16 (7), 2355-2367 (2010).</li><li>Wang, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Osteogenesis+and+angiogenesis+of+tissue-engineered+bone+constructed+by+prevascularized+&#946;-tricalcium+phosphate+scaffold+and+mesenchymal+stem+cells.">Osteogenesis and angiogenesis of tissue-engineered bone constructed by prevascularized &#946;-tricalcium phosphate scaffold and mesenchymal stem cells.</a> Biomaterials. 36, 9452-9461 (2010).</li><li>Cuadra, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+results+of+burned+hands+treated+with+Integra.">Functional results of burned hands treated with Integra.</a> Journal of Plastic, Reconstructive &amp; Aesthetic Surgery. 65 (2), 228-234 (2012).</li><li>Wilcke, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=VEGF(165)+and+bFGF+protein-based+therapy+in+a+slow+release+system+to+improve+angiogenesis+in+a+bioartificial+dermal+substitute+in+vitro+and+in+vivo.">VEGF(165) and bFGF protein-based therapy in a slow release system to improve angiogenesis in a bioartificial dermal substitute in vitro and in vivo.</a> Langenbecks Arch Surg. 392 (3), 305-314 (2007).</li><li>Condurache, A., Aach, T., Grzybowsky, S., Machens, H. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vessel+segmentation+and+analysis+in+laboratory+skin+transplant+micro-angiograms.">Vessel segmentation and analysis in laboratory skin transplant micro-angiograms.</a> Proceedings of the Eighteenth IEEE Symposium on Computer-Based Medical Systems. , 21-26 (2005).</li><li>Danner, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+human+sweat+gland-derived+stem+cells+for+enhancing+vascularization+during+dermal+regeneration.">The use of human sweat gland-derived stem cells for enhancing vascularization during dermal regeneration.</a> Journal of Investigative Dermatology. 132 (6), 1707-1716 (2012).</li><li>Shaterian, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real+Time+Analysis+of+the+Kinetics+of+Angiogenesis+and+Vascular+Permeability+in+an+Animal+Model+of+Wound+Healing.">Real Time Analysis of the Kinetics of Angiogenesis and Vascular Permeability in an Animal Model of Wound Healing.</a> Burns. 35 (6), 811-817 (2009).</li><li>McDonald, D. M., Choyke, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+of+angiogenesis:+from+microscope+to+clinic.">Imaging of angiogenesis: from microscope to clinic.</a> Nature Medicine. 9 (6), 713-725 (2003).</li><li>Bergeron, L., Tang, M., Morris, S. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+vascular+injection+techniques+for+the+study+of+perforator+flaps.">A review of vascular injection techniques for the study of perforator flaps.</a> Plastic and Reconstructive Surgery. 117, 2050-2057 (2006).</li><li>Schlatter, P., K&#246;nig, M. F., Karlsson, L. M., Burri, P. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+study+of+intussusceptive+capillary+growth+in+the+chorioallantoic+membrane+(CAM)+of+the+chicken+embryo.">Quantitative study of intussusceptive capillary growth in the chorioallantoic membrane (CAM) of the chicken embryo.</a> Microvascular Research. 54 (1), 65-73 (1997).</li><li>Lehr, H. A., Leunig, M., Menger, M. D., Nolte, D., Messmer, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dorsal+skinfold+chamber+technique+for+intravital+microscopy+in+nude+mice.">Dorsal skinfold chamber technique for intravital microscopy in nude mice.</a> American Journal of Pathology. 143 (4), 1055-1062 (1993).</li><li>Menger, M. D., J&#228;ger, S., Walter, P., Hammersen, F., Messmer, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+technique+for+studies+on+the+microvasculature+of+transplanted+islets+of+Langerhans+in+vivo.">A novel technique for studies on the microvasculature of transplanted islets of Langerhans in vivo.</a> International journal of microcirculation, clinical and experimental. 9 (1), 103-117 (1990).</li><li>Laschke, M. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+spheroids+of+adipose-derived+mesenchymal+stem+cells+are+potent+initiators+of+blood+vessel+formation+in+porous+polyurethane+scaffolds.">Three-dimensional spheroids of adipose-derived mesenchymal stem cells are potent initiators of blood vessel formation in porous polyurethane scaffolds.</a> Acta Biomaterials. 9 (6), 6876-6884 (2013).</li><li>Ega&#241;a, J. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+human+mesenchymal+cells+to+improve+vascularization+in+a+mouse+model+for+scaffold-based+dermal+regeneration.">Use of human mesenchymal cells to improve vascularization in a mouse model for scaffold-based dermal regeneration.</a> Tissue Eng Part A. 15 (5), 1191-1200 (2009).</li><li>Condurache, A., Aach, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vessel+segmentation+in+angiograms+using+hysteresis+thresholding.">Vessel segmentation in angiograms using hysteresis thresholding.</a> Proceedings of the Ninth IAPR Conference on Machine Vision Applications. , 269-272 (2005).</li><li>Ega&#241;a, J. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ex+vivo+method+to+visualize+and+quantify+vascular+networks+in+native+and+tissue+engineered+skin.">Ex vivo method to visualize and quantify vascular networks in native and tissue engineered skin.</a> Langenbecks Archives of Surgery. 394, 349-356 (2009).</li></ol>

有必要建立在改善血流灌注组织工程构建，这需要新的可靠的方法发展到生物材料研究中的血管形成过程中的成功做法。用于制造支架的血管的体外可见常见的方法包括使用显微镜，它提供了高分辨率的工具。在大多数情况下，虽然，这种方法仅限于较小的组织区域的分析，而且往往是昂贵的和费时的。此外，它通常包括复杂的标签和染色的方法，其中血液灌注容器不能从非功能性的人<sup...

在最近的几十年中，组织工程开辟了新的治疗选择与人体自身的细胞替换1组织缺损。为了支持组织再生的生理过程，支架被设计成可生物降解的结构，即提供了一种方案，其中从伤口床的细胞能生长和恢复的缺陷2,3。 血管化不足被认为是主要的障碍，这阻碍了生物人工支架4的临床突破。与细胞的向内生长，营养物质和氧的增加，材料的血管化的需求变得至关重要。因此不足或延迟血管可导致组织工程产品5中央坏死。此外，血管提供免疫感受态细胞并去除代谢残留在再生区。高感染率和低再生只部分的血流灌注不足，在组织工程中所观察到的结果，其目的都是为了通过增加支架6,7的血管被避免。 几种策略，目的是改善血管聚焦在生物材料本身和支架的微观结构中的关键作用。有深入的研究努力，从修理转向愈合过程中再生 ，从而（重新）产生的组织与最接近的生理特性，以一个开发新的方法来恢复8,9。到研究和评估与问候他们的再生潜能的生物材料包括胶原蛋白，纤维蛋白，壳聚糖和海藻酸钠10,11。这些生物材料可以使用，组合为骨干，建立使用不同的策略，新的支架，如脱细胞组织，自组装，快速成型和静电12。为了ENHANCE人体自身的再生能力，支架可生物活化。重组血管生成生长掺入因子13或基因载体等因素后14编码已经显示出改善支架的血管。利用干细胞已被广泛证明是一种很有前途的战略，以改善血管化，其中的间充质基质细胞和内皮祖细胞获得了最多的关注15,16。其他方法试图建立包含移植前17预制的血管网络结构。尽管支架设计密集的努力和他们的生物活性，没有战略已经在临床上显著水平提高血管，并与大规模烧伤除真皮替代的，翻译生物工程材料进入临床常规只发生欲言又止18 。 之一的原因的血管人工组织结构仍然是一个未解决的问题，是评价新技术在体内的方法取得成功的难度。虽然在体外实验可提供支架的血管形成潜力的重要的见解，合适的动物模型是必需的，研究的关键参数，如材料的生物相容性，该处理的特别重要的安全性和有效性，并且，该组织的血管构建。因此，可靠的工具来可视化和量化的血管网络， 在体内是必需的。 在本研究中，我们提出了一种简单而可靠的方法，它允许取出的支架内的血管网络的可视化和量化。这个方法是基于组织透和数字分割。由于该方法是非侵入性的，它可以使靶材料的进一步分子和组织学分析。

<table border="0" cellpadding="0" cellspacing="0" width="1091">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:319px;">Name of Material/ Equipment</td>
			<td style="width:223px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:431px;">Comments/Description</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:319px;">Ethilon P-3 13 mm 3/8 circle 5-0</td>
			<td style="width:223px;">Ethicon, Norderstedt, Germany</td>
			<td style="width:119px;">698G</td>
			<td>Ethilon polyamid-6 precision point-reverse cutting suture</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:319px;">Biopsy punches (10 mm)</td>
			<td style="width:223px;">Xiomedics, Acuderm inc., Fort Lauderdale, FL, USA</td>
			<td style="width:119px;">P1050</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:319px;">Biopsy punches (12 mm)</td>
			<td style="width:223px;">Xiomedics, Acuderm inc., Fort Lauderdale, FL, USA</td>
			<td style="width:119px;">P1250</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Digital camera&#160;</td>
			<td style="width:223px;">Ricoh, Hannover, Germany</td>
			<td style="width:119px;">Cx1</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Gazin Mullkompresse&#160;</td>
			<td style="width:223px;">Lohmann und Rauscher, Neuwied, Germany</td>
			<td style="width:119px;">13622</td>
			<td>Sterile gauze (10 cm x 10 cm)</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Double-layer collagen-based scaffold (8 &#39;x 10&#39;)</td>
			<td style="width:223px;">Integra Life Science Corporation, Plainsboro, NJ, USA</td>
			<td style="width:119px;">88101</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Isoflurane, liquid-gas for inhalative anesthesia&#160;</td>
			<td style="width:223px;">Baxter, Unterschleissheim, Germany</td>
			<td style="width:119px;">100196040</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Pentobarbital, 16 g / 100 ml</td>
			<td style="width:223px;">Fa. Merial, Hallbergmoos</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:319px;">Nuri Nu/Nu Nude mice, CrLNU-Foxn1nu</td>
			<td style="width:223px;">Charles River, Sulzfeld, Germany</td>
			<td style="width:119px;">Strain code 088</td>
			<td>Athymic nude mice, 6 to 8 weeks of age and with a body weight between 20 to 25 g&#160;</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:319px;">Buprenorphine (0.3 mg/ml)</td>
			<td style="width:223px;">Essex Pharma GmbH, Munich, Germany</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Titanized mesh (15 cm x 15 cm), extralight</td>
			<td style="width:223px;">PFM Medical AG, K&#246;ln, Germany</td>
			<td style="width:119px;">6000029</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:319px;">Tissucol Duo S Immuno 2 ml</td>
			<td style="width:223px;">Baxter Germany GmbH, Unterschlei&#223;heim, Germany</td>
			<td style="width:119px;">B1332020110614</td>
			<td>Fibrin-thrombin solution&#160;</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Transparent adhesove drape (30.5 cm x 26 cm)</td>
			<td style="width:223px;">KCI Medical Products, Wimborne Dorset, UK</td>
			<td style="width:119px;">M6275009/10</td>
			<td></td>
		</tr>
	</tbody>
</table>

a full skin defect model to evaluate vascularization of biomaterials in vivo

血管化不足被认为是限制性的组织工程化构建体的临床成功的主要因素之一。为了评价新的策略，其目的是提高血管化的，可靠的方法是必需的，以使得在生长的新血管进入生物人工支架可见和量化的结果。在过去的几年中，我们的团队已经推出了全层皮肤缺损模型，使血管的直接可视化通过透，并提供量化，通过数字化分割的可能性。在此模式中，一个外科地在小鼠背部创建完整皮肤缺陷并将它们替换为所测试的材料。分子或感兴趣的细胞也可以在这些材料掺入以研究它们的潜在影响。经过自己选择的观察时间，材料植进行评估。双侧伤口提供进行内部比较次的可能在减少个体之间的工件，以及减少所需的研究的动物的数量的。相较于其他方法，我们的方法提供了一种简单，可靠和具有成本效益的分析。我们已经实现了这个模式作为常规工具测试不同的生物材料和生物活化方法的血管时，执行高解析度的筛选。

一个可靠的双边全皮肤缺损所用的小鼠（ 图1），其中所述皮肤可以由生物材料进行研究（ 图2）来代替被创建。在这里，没有严重的并发症是中或手术操作后，无论是宏观的感染或异物反应的迹象观察。在极少数情况下，支架被当鼠标中删除丢失。伤口收缩从未被观察到（ 图3）。组织透允许明确的高达30微米的宽度的血管结构的可视化，整个组织样品，其?...

Watch this Scientific Journal Video about A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo at JoVE.com

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

血管是关键，成功的组织工程方法。因此，可靠的技术是必需的，以评估组织构建体的血管网络的发展。在这里，我们提出一个简单和具有成本效益的方法来可视化和量化血管在体内 。

一个完整的皮肤缺损模型，以评估生物材料的血管化<em&gt;体内</em

Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to ...

Research

JoVE Journal

Bioengineering

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal  Skin and Melanoma Progression

三维人体皮肤重建模型：一种工具来研究正常皮肤恶性黑色素瘤进展

Most in vitro studies in experimental skin biology have been done in 2-dimensional (2D) monocultures, while accumulating evidence suggests that cells ...

科研

生物工程

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

循环压力为生物反应器的设计体外主动脉心脏瓣膜的研究

The aortic valve, located between the left ventricle and the aorta, allows for unidirectional blood flow, preventing backflow into the ventricle. Aortic ...

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

三维全层皮肤的生成等效和自动伤人

In vitro models are a cost effective and ethical alternative to study cutaneous wound healing processes. Moreover, by using human cells, these models ...

Sandwich-like Microenvironments to Harness Cell/Material Interactions

三明治般的微环境来驾驭细胞/材料相互作用

Cell culture has been traditionally carried out on bi-dimensional (2D) substrates where cells adhere using ventral receptors to the biomaterial surface. ...

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

在组织工程由内在血管化<em&gt;在体内</em&gt;组织工程商会

In reconstructive surgery, there is a clinical need for an alternative to the current methods of autologous reconstruction which are complex, costly and ...

A Method for Evaluating Timeliness and Accuracy of Volitional Motor Responses to Vibrotactile Stimuli

一种意志的运动反应的及时性和准确性，以振动触觉刺激评价方法

Artificial sensory feedback (ASF) systems can be used to compensate for lost proprioception in individuals with lower-limb impairments.  Effective design ...

Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics

在皮肤扩张使用多视点立体和Isogeometric运动学的猪模型应变的定量

Tissue expansion is a popular technique in plastic and reconstructive surgery that grows skin in vivo for correction of large defects such as burns and ...

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

多显微镜下的血管生成术在基因修饰的 3 d-PLGA/nHAp 支架上进行颅骨临界骨缺损修复

The reconstruction of critically sized bone defects remains a serious clinical problem because of poor angiogenesis within tissue-engineered scaffolds ...

A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection

A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection

一种用于生物技术相关金黄色葡萄球菌感染活体可视化和内膜分析的斑马鱼胚胎模型

Biomaterial-associated infection (BAI) is a major cause of the failure of biomaterials/medical devices. Staphylococcus aureus is one of the major ...

A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator

一种可靠、可重复的可重复的大鼠部分股骨缺陷模型

Orthopedic research relies heavily on animal models to study mechanisms of bone healing in vivo as well as investigate the new treatment techniques. ...