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DOI :
10.3791/51433-v
May 12th, 2014
Chapters
0:05
Title
1:34
Isolation and Culturing of Adult Human Brain Cells
2:55
In vitro Expansion and Characterization of Adult Human Brain Cells
5:15
Retroviral Transduction of Pericyte-derived Cells and Reprogramming into Induced Neurons
6:41
Results: Reprogramming Adult Brain Cells into Induced Neurons
9:11
Conclusion
直接の系譜 - 再プログラミングのための脳常駐細胞を標的とすることは、脳の修復のための新たな視点を提供しています。ここでは、成人の大脳皮質から脳常駐周皮細胞について濃縮文化を準備し、転写因子Sox2のとASCL1のレトロウイルス媒介発現によって誘導神経細胞にこれらを変換する方法のプロトコルについて説明します。
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