Name: determination of the spontaneous locomotor activity in drosophila melanogaster
Uploaded: 2014-04-10T16:00:00
Description: Watch this Scientific Journal Video about Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster at JoVE.com

这项工作是由美国国立卫生研究院（AG023088为BR）的资助。

注：广-S品系是从布卢明顿库存中心获得的标准野生型背景线。 1，食品制作和配方1000毫升食物注意：本节介绍的协议准备食物。大型金属罐是用来在同一时间准备大约18L的食物。这里所描述的协议的小型化，并使用千毫升H 2 O的食品灭菌两次。 <ol><li>混合113克蔗糖和28克啤酒酵母643毫升的水。留下的热板集配料，在25℃下用搅拌棒混合在整个15?...

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USA. 100 (6), 3369-3372 (2003).</li><li>Grover, D., Yang, J., Tavar&#233;, S., Tower, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+tracking+of+fly+movement+and+gene+expression+using+GFP.">Simultaneous tracking of fly movement and gene expression using GFP.</a> BMC Biotechnol. 8, 93 (2008).</li><li>Grover, D., Yang, J., Tavar&#233;, S., Tower, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+tracking+of+movement+and+gene+expression+in+multiple+Drosophila+melanogaster+flies+using+GFP+and+DsRED+fluorescent+reporter+transgenes.">Simultaneous tracking of movement and gene expression in multiple Drosophila melanogaster flies using GFP and DsRED fluorescent reporter transgenes.</a> BMC Res Notes. 2 (58), 1-11 (2009).</li><li>Ardekani, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+tracking+and+behaviour+monitoring+of+multiple+fruit+flies.">Three-dimensional tracking and behaviour monitoring of multiple fruit flies.</a> J. 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Methods. 202 (1), 45-52 (2011).</li><li>Parashar, V., Rogina, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=dSir2+mediates+the+increased+spontaneous+physical+activity+in+flies+on+calorie+restriction.">dSir2 mediates the increased spontaneous physical activity in flies on calorie restriction.</a> Aging. 1 (6), 529-541 (2009).</li><li>Kaneuchi, T., Togawa, T., Matsuo, T., Fuyama, Y., Aigaki, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficient+measurement+of+H2O2+resistance+in+Drosophila+using+an+activity+monitor.">Efficient measurement of H2O2 resistance in Drosophila using an activity monitor.</a> Biogerontology. 4 (3), 157-165 (2003).</li><li>Carey, J. 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Gerontol. 43 (8), 739-749 (2008).</li><li>Osterwalder, T., Yoon, K. S., White, B. H., Keshishian, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+conditional+tissue-specific+transgene+expression+system+using+inducible+GAL4.">A conditional tissue-specific transgene expression system using inducible GAL4.</a> Proc. Natl. Acad. Sci. USA. 98 (22), 12596-12601 (2001).</li><li>Dietzl, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+genome-wide+transgenic+RNAi+library+for+conditional+gene+inactivation+in+Drosophila.">A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.</a> Nature. 448 (7150), 151-156 (2007).</li><li>Chiu, J. C., Low, K. H., Pike, D. 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苍蝇的自发活动是由许多因素，如年龄，遗传背景，性别和影响2,13,18,19。此外，环境因素，诸如食物，温度环境，除了不同的药物，和白天/黑夜光周期的卡路里含量可影响蝇的活性。例如，雄性苍蝇同龄具有更高的自发运动量相比，女性（ 图1）。因此，苍蝇相同年龄和性别的，应相互比较。当检验遗传操作上飞活性，如过表达或特定基因的功能丧失的效果，实验组和对照?...

果蝇， 果蝇 ，已被用来作为一种有价值的模式生物来研究相关 ​​复杂的行为，如学习和记忆，社会交往，侵略，滥用药物，睡眠，感觉功能，求偶，交配和1,2机制。已通过多种协议研究的一个行为是自发活动。负趋地性是用于测量果蝇活动开发了第一种方法，而这种协议包括测量苍蝇的那个到达小瓶的一定高度后的苍蝇都摇动到容器1,3的底部的百分比。此方法具有简单，廉价的优点，并且由于它不要求它能够在任何实验室中进行任何特殊的设备。它已被用来作为一种有价值的筛查工具，研究苍蝇的流动性3种不同的基因操作的效果。但是，它是时间和劳动力密集型的一ND有偏见的，由于小瓶和人类录音变量晃动的可能性。 在由快速迭代负趋地性（环）方法4,5，这需要飞瓶以下的苍蝇晃动底部照片的发展负趋地性方法进行了改进。本协议的优点是它的灵敏度和测试大量蝇小瓶在同一时间的可能性。然而，该协议仍具有人为错误的可能性，并只测量负趋地性。其他实验室使用了简单的观察在培养瓶，以确定自主活动6。 最近已经开发了几种视频记录系统，用于测量苍蝇的运动行为。一个视频监控协议提供时间记录7之前的调整。通过Slawson 等人描述的方法。还使用一个空气脉冲停止movemen吨，直到记录的开始，这有可能是应激对动物7。这种方法提供的平均速度，最大速度，时间花费在运动等。另一个三维跟踪系统测量个体的苍蝇在约0.2秒内自由飞行起飞8的最大速度信息。一种三维视频监控协议使用果蝇表达GFP和装有过滤器，允许荧光的检测来确定飞流动性9多台摄像机。在这个协议苍蝇往往表现出由于果蝇文化的形状圆柱形飞行模式，这是潜在的小瓶10。这种方法是通过使用一个圆顶，使测量两只苍蝇11的自发运动改善。使用相机来自动监控和量化果蝇的个人和社会行为的高通量方法一直还描述12。 Zou 等人开发，它使用两个电脑辅助相机记录一生的行为和动作，如静止，移动，飞行，吃，喝，或个别实蝇果实蝇死亡13一个行为监控系统（BMS）。几个其它视频系统已被开发并监控蝇行为活动14,15。 在这里，我们描述了量化果蝇的活动，利用人口监测的方法。这些监视器都装在温度和湿度控制孵化器在25°C下12小时昼夜光周期。每个群体都有监视器放置在环定位在3个不同高度的红外光束。每次一飞跨环移动它中断了红外线光束，其上记录由一个微处理器独立地记录和计数小瓶内蝇的活性。微处理器上传小瓶内的总活动的电脑用户定义intervaLS，可以从1秒至60分钟。这里介绍的方法提供充足的时间过得来适应新的环境，并允许苍蝇多达120人口的自发活动的同时测量。此外，我们描述准备食物，飞维护，设置在温度控制恒温箱的流动性人口的显示器，以及可能影响业绩的潜在因素。该方法可用于研究不同环境或遗传修改如何影响蝇自发活动。

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>Sucrose FCC Food Grade 100 LB,</td>
			<td>Fisher Scientific MP Biomedicals</td>
			<td>ICN90471380</td>
			<td></td>
		</tr>
		<tr>
			<td>Brewer&#8217;s Yeast</td>
			<td>Fisher Scientific MP Biomedicals</td>
			<td>ICN90331280</td>
			<td></td>
		</tr>
		<tr>
			<td>Drosophila Agar Fine</td>
			<td>SciMart</td>
			<td>DR-820-25F</td>
			<td></td>
		</tr>
		<tr>
			<td>Cornmeal</td>
			<td>Fisher Scientific MP Biomedicals</td>
			<td>ICN90141125</td>
			<td></td>
		</tr>
		<tr>
			<td>Methyl4-hydroxybenzoate, tegosept</td>
			<td>Sigma</td>
			<td>H5501-5KG</td>
			<td></td>
		</tr>
		<tr>
			<td>EtOH</td>
			<td>Pharmco-AAPER</td>
			<td>111000200</td>
			<td></td>
		</tr>
		<tr>
			<td>Active Dry Yeast</td>
			<td>Fisher Scientific</td>
			<td>ICN10140001</td>
			<td></td>
		</tr>
		<tr>
			<td>Fly CO2 pad</td>
			<td>LabScientific</td>
			<td>BGSU-7</td>
			<td></td>
		</tr>
		<tr>
			<td>Stereo Microscope</td>
			<td>Olympus</td>
			<td>SZ40</td>
			<td></td>
		</tr>
		<tr>
			<td>Drosophila carbon dioxide (CO2) tank</td>
			<td>Airgas</td>
			<td>UN1013</td>
			<td></td>
		</tr>
		<tr>
			<td>Small paint brush for pushing the flies</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Shell vial wide</td>
			<td>Fischer Scientific</td>
			<td>AS519</td>
			<td></td>
		</tr>
		<tr>
			<td>Buzzplugs for wide plastic vials</td>
			<td>Fischer Scientific</td>
			<td>AS275</td>
			<td></td>
		</tr>
		<tr>
			<td>Glass vials (25x95mm)</td>
			<td>Fischer Scientific Kimble 60931-8</td>
			<td>AS-574</td>
			<td></td>
		</tr>
		<tr>
			<td>Sponge plugs for glass vials</td>
			<td>SciMart</td>
			<td>DR-750</td>
			<td></td>
		</tr>
		<tr>
			<td>Drosophila Food Dispenser</td>
			<td>Applied Scientific (Fischer Scientific)</td>
			<td>AS780Q</td>
			<td></td>
		</tr>
		<tr>
			<td>DPM Drosophila Population Monitor</td>
			<td>Trikinetics Inc.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>DC Power Supply with line cord</td>
			<td>Trikinetics Inc.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>PSIU9 The Power Supply Interface Unit</td>
			<td>Trikinetics Inc.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Telephone cables and 5 way splitters</td>
			<td>Trikinetics Inc.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Universal Serial Bus (USB) hardware</td>
			<td>Trikinetics Inc.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Macintosh or Windows PC with UCB port</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>DAMSystem308X Data Acquisition Software for Macintoch OSX (Intel)</td>
			<td>www.trikinetics.com</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>DAMSystem308 Data Acquisition Software for Windows PC (XP/Vista/7)</td>
			<td>www.trikinetics.com</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>DAMFileScan108X software for Macintosh</td>
			<td>www.trikinetics.com</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>DAMFileScan108X software for Windows PC (XP/Vista/7)</td>
			<td>www.trikinetics.com</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>USB software (PSIUdrivers.zip)</td>
			<td>www.trikinetics.com</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>DAMSystem Notes 308</td>
			<td><a href="http://www.trikinetics.com/Downloads/DAMSystem%20Notes%20308.pdf">(http://www.trikinetics.com/Downloads/DAMSystem%20Notes%20308.pdf</a></td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

determination of the spontaneous locomotor activity in drosophila melanogaster

果蝇已被用来作为一个极好的模型有机体，以研究影响行为环境和遗传操作。一个这样的行为是自发活动。在这里，我们描述了我们的协议，它利用果蝇种群监测和跟踪系统，可连续监测苍蝇的自发活动的好几天的时间。该方法简单，可靠，客观和可以用来检查老化，性别，改变食物的卡路里含量，另外的药物，或模仿人类疾病的遗传操作的效果。

<html>在果蝇的自发活动依赖于飞性别（ 图3A），食品（ 图3B）的卡路里含量和光/暗周期。一旦灯被关闭飞活性显着降低。 图3A示出24小时的男性和女性的苍蝇自发活动记录。在x轴以星号标记当光被关掉的时间和过渡到暗循环。 图3B示出收集在三个群体监视器男性的平均自发活动之间的标准偏差拥有3日龄玉米食品。收集在24小时内自发运动?...

Watch this Scientific Journal Video about Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster at JoVE.com

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

果蝇是研究影响的行为，如自发活动的遗传或环境的操作非常有用。在这里，我们描述了一个协定，它利用监测与红外光束和数据分析软件来量化自发活动。

在自发活动的测定<em&gt;果蝇</em

Drosophila melanogaster has been used as an excellent model organism to study environmental and genetic manipulations that affect behavior. One such ...

Research

JoVE Journal

Neuroscience

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

在昆虫单感器记录果蝇按蚊</em

The sense of smell is essential for insects to find foods, mates, predators, and oviposition sites3. Insect olfactory sensory neurons (OSNs) are enclosed ...

科研

神经科学

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

含量自发活动，研究昼夜节律和睡眠参数果蝇</em

Most life forms exhibit daily rhythms in cellular, physiological and behavioral phenomena that are driven by endogenous circadian (&equiv;24 hr) ...

Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration

含量的运动能力，学习和记忆的赤字果蝇</em神经退行性疾病>模型

Advances in genetic methods have enabled the study of genes involved in human neurodegenerative diseases using Drosophila as a model system1. Most of ...

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

标记的单细胞在中枢神经系统<em&gt;果蝇</em

In this  article we describe how to individually label neurons in the embryonic CNS of Drosophila melanogaster by juxtacellular injection of the ...

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic刺激的逃避行为<em&gt;果蝇</em

A  growing number of genetically encoded tools are becoming available that allow non-invasive  manipulation of the neural activity of specific neurons in ...

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

光遗传学的扰动激光照射半完好的神经活动<em&gt;果蝇</em&gt;幼虫运动

Drosophila larval locomotion is a splendid model system in developmental and physiological neuroscience, by virtue of the genetic accessibility of the ...

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster

简单的检测方法的社交回避的量化<em&gt;果蝇</em

Drosophila melanogaster is an emerging model to study different aspects of social interactions. For example, flies avoid areas previously occupied by ...

Conditions Affecting Social Space in Drosophila melanogaster

Conditions Affecting Social Space in Drosophila melanogaster

在条件影响社会空间<em&gt;果蝇</em

The social space assay described here can be used to quantify social interactions of Drosophila melanogaster &#8212; or other small insects &#8212; in a ...

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

一个行为分析对机械感觉MARCM为基础的克隆<em&gt;果蝇</em

Because of the structural and functional homology to the hair cells of the mammalian inner ear, the neurons that innervate the Drosophila external sense ...

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

一个简单的方法来衡量在改建悬赏寻求行为使用<em&gt;果蝇</em

We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We ...