Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/51455-v
•
10:56 min
May 17th, 2014
Chapters
0:05
Title
1:54
Preparation of Sucrose Gradient
3:38
Isolation and Sedimentation of Polysomes
6:59
Polysome Fractionation and RNA Extraction
9:22
Results: Analytics of Translational Activity in MCF7 Cells Using Polysome Fractionation
10:24
Conclusion
核糖体蛋白质合成中发挥核心作用。用蔗糖密度梯度离心多核糖体(核糖体)分离可直接测定在基因组范围内个人mRNA的翻译效率。此外,可用于核糖体和多核糖体相关的因素,例如伴侣分子和信号转导分子生化分析此方法。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved