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DOI :
10.3791/51458-v
•
12:47 min
March 20th, 2014
Chapters
0:05
Title
1:36
Acute Hippocampal Slice Preparation
4:58
Bolus Loading of Astrocytes with Ca2+ Indicator
7:22
Recording Spontaneous and Gq G Protein-coupled Receptor Agonist-evoked Astrocytic Ca2+ Activity in Hippocampal Slices
9:59
Results
11:48
Conclusion
在这里,我们描述了用于诱导稳态可塑性神经元星形胶质细胞的G蛋白偶联受体的可塑性研究方案的适应。最近用于检查变化星形细胞组I型mGluRs在幼年小鼠中,该方法可以应用于测量各种星形细胞G蛋白偶联受体的比例,在从成年小鼠原位和体内组织,并更好地欣赏星形细胞受体的敏感性的变化的神经元活性。
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